Department of Bioengineering, Rice University , Houston, Texas.
Tissue Eng Part A. 2013 Dec;19(23-24):2565-76. doi: 10.1089/ten.TEA.2013.0256. Epub 2013 Aug 2.
In this work, human mesenchymal stem cells (hMSCs) and their osteogenically precultured derivatives were directly cocultured with human umbilical vein endothelial cells (HUVECs) on electrospun three-dimensional poly(ɛ-caprolactone) microfiber scaffolds to evaluate the coculture's effect on the generation of osteogenic constructs. Specifically, cells were cultured on scaffolds for up to 3 weeks, and the cellularity, alkaline phosphatase (ALP) activity, and bone-like matrix formation were assessed. Constructs with cocultures and monocultures had almost identical cellularity after the first week, however, lower cellularity was observed in cocultures compared to monocultures during the subsequent 2 weeks of culture. Scaffolds with cocultures showed a significantly higher ALP activity, glycosaminoglycan and collagen production, as well as greater calcium deposition over the course of study compared to monocultures of hMSCs. Furthermore, the osteogenic outcome was equally robust in cocultures containing osteogenically precultured and non-precultured hMSCs. The results demonstrate that the combination of MSC and HUVEC populations within a porous scaffold material under osteogenic culture conditions is an effective strategy to promote osteogenesis.
在这项工作中，人骨髓间充质干细胞（hMSCs）及其预先成骨培养的衍生物直接与人体脐静脉内皮细胞（HUVECs）共培养在静电纺丝三维聚（ε-己内酯）微纤维支架上，以评估共培养对成骨构建体生成的影响。具体而言，细胞在支架上培养长达 3 周，评估细胞数量、碱性磷酸酶（ALP）活性和类骨质基质形成。第 1 周后，共培养和单培养的构建体具有几乎相同的细胞数量，但在随后的 2 周培养过程中，共培养的细胞数量明显低于单培养。与 hMSC 的单培养相比，共培养的支架在研究过程中显示出更高的碱性磷酸酶（ALP）活性、糖胺聚糖和胶原蛋白的产生以及更高的钙沉积。此外，在成骨培养条件下，含有预先成骨培养和非培养的 hMSCs 的共培养物的成骨结果同样强大。研究结果表明，在成骨培养条件下，将 MSC 和 HUVEC 群体组合在多孔支架材料中是促进成骨的有效策略。
