Departments of Biomedical Engineering, University of Minnesota, Minneapolis, MN, USA.
Exp Cell Res. 2013 Oct 1;319(16):2409-17. doi: 10.1016/j.yexcr.2013.06.006. Epub 2013 Jun 22.
In vitro models of endothelial assembly into microvessels are useful for the study of angiogenesis and vasculogenesis. In addition, such models may be used to provide the microvasculature required to sustain engineered tissues. A large range of in vitro models of both angiogenesis and vasculogenesis have utilized fibrin gel as a scaffold. Although fibrin gel is conducive to endothelial assembly, its ultrastructure varies substantially based on the gel formulation and gelation conditions, making it challenging to compare between models. This work reviews existing models of endothelial assembly in fibrin gel and posits that differerences between models are partially caused by microstructural differences in fibrin gel.
体外模型用于研究血管生成和血管发生。此外，此类模型可用于提供维持工程组织所需的微血管。大量的血管生成和血管发生的体外模型都利用纤维蛋白凝胶作为支架。尽管纤维蛋白凝胶有利于内皮细胞的组装，但它的超微结构因凝胶配方和凝胶条件的不同而有很大差异，这使得模型之间的比较变得具有挑战性。本文综述了纤维蛋白凝胶中内皮细胞组装的现有模型，并提出模型之间的差异部分是由于纤维蛋白凝胶的微观结构差异引起的。
