Kim Soo Min, Kim Jinmyeong, Noh Seungwoo, Sohn Hiesang, Lee Taek
Department of Chemical Engineering, Kwangwoon University, 20 Kwangwoon-ro, Nowon-gu, Seoul, 01899 Republic of Korea.
Biochip J. 2020;14(4):327-339. doi: 10.1007/s13206-020-4401-2. Epub 2020 Nov 16.
In nowadays, we have entered the new era of pandemics and the significance of virus detection deeply impacts human society. Viruses with genetic mutations are reported nearly every year, and people have prepared tools to detect the virus and vaccines to ensure proper treatments. Influenza virus (IV) is one of the most harmful viruses reporting various mutations, sub-types, and rapid infection speed for humans and animals including swine and poultry. Moreover, IV infection presents several harmful symptoms including cough, fever, diarrhea, chills, even causing death. To reduce the IV-induced harm, its proper and rapid detection is highly required. Conventional techniques were used against various IV sub-types including H1N1, H3N2, and H5N1. However, some of the techniques are time-consuming, expensive, or labor-intensive for detecting IV. Recently, the nucleic acid-based aptamer has gained attention as a novel bioprobe for constructing a biosensor. In this review, the authors discuss the recent progress in aptasensors for detecting IV in terms of an electrochemical and an optical biosensor.
如今，我们已进入大流行的新时代，病毒检测的意义对人类社会产生了深远影响。几乎每年都会报告出现基因突变的病毒，人们也已准备好检测病毒的工具和疫苗以确保得到妥善治疗。流感病毒（IV）是最具危害性的病毒之一，它具有多种突变、亚型，并且对包括猪和家禽在内的人类和动物的感染速度很快。此外，IV感染会出现多种有害症状，包括咳嗽、发烧、腹泻、发冷，甚至会导致死亡。为了减少IV造成的危害，迫切需要对其进行准确、快速的检测。传统技术被用于检测包括H1N1、H3N2和H5N1在内的各种IV亚型。然而，其中一些技术在检测IV时耗时、昂贵或劳动强度大。最近，基于核酸的适配体作为构建生物传感器的新型生物探针受到了关注。在这篇综述中，作者从电化学生物传感器和光学生物传感器的角度讨论了用于检测IV的适配体传感器的最新进展。
