Pitchakarn Pornsiri, Chewonarin Teera, Ogawa Kumiko, Suzuki Shugo, Asamoto Makoto, Takahashi Satoru, Shirai Tomoyuki, Limtrakul Pornngarm
Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Asian Pac J Cancer Prev. 2013;14(5):2859-63. doi: 10.7314/apjcp.2013.14.5.2859.
Polyphenolic compounds from pomegranate fruit extracts (PFEs) have been reported to possess antiproliferative, pro-apoptotic, anti-inflammatory and anti-invasion effects in prostate and other cancers. However, the mechanisms responsible for the inhibition of cancer invasion remain to be clarified. In the present study, we investigated anti-invasive effects of ellagic acid (EA) in androgen-independent human (PC-3) and rat (PLS10) prostate cancer cell lines in vitro. The results indicated that non-toxic concentrations of EA significantly inhibited the motility and invasion of cells examined in migration and invasion assays. The EA treatment slightly decreased secretion of matrix metalloproteinase (MMP)-2 but not MMP-9 from both cell lines. We further found that EA significantly reduced proteolytic activity of collagenase/gelatinase secreted from the PLS-10 cell line. Collagenase IV activity was also concentration-dependently inhibited by EA. These results demonstrated that EA has an ability to inhibit invasive potential of prostate cancer cells through action on protease activity.
据报道，石榴果实提取物（PFEs）中的多酚类化合物在前列腺癌和其他癌症中具有抗增殖、促凋亡、抗炎和抗侵袭作用。然而，抑制癌症侵袭的机制仍有待阐明。在本研究中，我们在体外研究了鞣花酸（EA）对雄激素非依赖性人前列腺癌细胞系（PC-3）和大鼠前列腺癌细胞系（PLS10）的抗侵袭作用。结果表明，无毒浓度的EA显著抑制了迁移和侵袭试验中所检测细胞的运动性和侵袭能力。EA处理使两种细胞系中基质金属蛋白酶（MMP）-2的分泌略有减少，但对MMP-9的分泌没有影响。我们进一步发现，EA显著降低了PLS-10细胞系分泌的胶原酶/明胶酶的蛋白水解活性。EA还浓度依赖性地抑制了IV型胶原酶的活性。这些结果表明，EA能够通过作用于蛋白酶活性来抑制前列腺癌细胞的侵袭潜能。
