Department of Gastroenterological Surgery, St. Luke's International Hospital, 9-1 Akashi-cho, Chuo-ku, Tokyo, 104-8560, Japan.
J Gastrointest Surg. 2013 Sep;17(9):1657-64. doi: 10.1007/s11605-013-2270-x. Epub 2013 Jun 27.
There have been very few detailed reports of the intestinal environment after surgical treatment for colorectal cancer (CRC). We analysed faecal microbiota, organic acids and pH to investigate the influence of colorectal surgery on the intestinal environment.
Faecal samples from 81 CRC patients were collected before the start of pre-operative preparation the day before surgery, as well as 7 days or more after surgery. Thirteen groups of intestinal microbiota, eight types of organic acids, and pH were measured using 16S rRNA-targeted reverse transcription-quantitative PCR, high-performance liquid chromatography and a pH meter, respectively.
Total bacterial counts (10.3 ± 0.6 vs. 9.4 ± 1.2 log10 cells/g; p < 0.001) and the numbers of 6 groups of obligate anaerobes were significantly decreased after surgery. In contrast, the populations of Enterobacteriaceae, Enterococcus, Staphylococcus and Pseudomonas were significantly increased. Post-operatively, the concentration of total organic acids was lower (77.9 ± 40.1 vs. 50.1 ± 37.0 μmol/g; p < 0.001) than the pre-operative concentration, and a significant reduction in short-chain fatty acids (SCFAs) was observed.
Significant changes in the intestinal environment, including marked decreases in obligate anaerobes, increases in pathogenic bacteria, and reductions in SCFAs, were detected after surgery for CRC.
关于结直肠癌（CRC）手术后的肠道环境，鲜有详细报道。我们分析粪便微生物群、有机酸和 pH 值，以研究结直肠手术对肠道环境的影响。
收集 81 例 CRC 患者手术前 1 天开始术前准备时，以及手术后 7 天或更长时间的粪便样本。使用 16S rRNA 靶向反转录定量 PCR、高效液相色谱和 pH 计分别检测 13 组肠道微生物群、8 种有机酸和 pH 值。
手术后总细菌计数（10.3 ± 0.6 与 9.4 ± 1.2 log10 细胞/g；p < 0.001）和 6 组专性厌氧菌数量明显减少。相比之下，肠杆菌科、肠球菌、葡萄球菌和假单胞菌的数量明显增加。术后，总有机酸浓度较低（77.9 ± 40.1 与 50.1 ± 37.0 μmol/g；p < 0.001），短链脂肪酸（SCFA）明显减少。
CRC 手术后肠道环境发生显著变化，包括专性厌氧菌明显减少、致病菌增加和 SCFA 减少。
