Nanotechnology Research Center and Department of Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
ACS Nano. 2013 Aug 27;7(8):6555-62. doi: 10.1021/nn305337c. Epub 2013 Jul 10.
Upon incorporation of nanoparticles (NPs) into the body, they are exposed to biological fluids, and their interaction with the dissolved biomolecules leads to the formation of the so-called protein corona on the surface of the NPs. The composition of the corona plays a crucial role in the biological fate of the NPs. While the effects of various physicochemical parameters on the composition of the corona have been explored in depth, the role of temperature upon its formation has received much less attention. In this work, we have probed the effect of temperature on the protein composition on the surface of a set of NPs with various surface chemistries and electric charges. Our results indicate that the degree of protein coverage and the composition of the adsorbed proteins on the NPs' surface depend on the temperature at which the protein corona is formed. Also, the uptake of NPs is affected by the temperature. Temperature is, thus, an important parameter that needs to be carefully controlled in quantitative studies of bionano interactions.
当纳米粒子 (NPs) 被引入体内时，它们会暴露于生物流体中，并且它们与溶解的生物分子的相互作用会导致 NPs 表面形成所谓的蛋白质冠。冠层的组成在 NPs 的生物命运中起着至关重要的作用。虽然已经深入探讨了各种物理化学参数对冠层组成的影响，但温度对其形成的作用却受到的关注较少。在这项工作中，我们研究了温度对一系列具有不同表面化学性质和电荷的 NPs 表面蛋白质组成的影响。我们的结果表明，蛋白质覆盖度和吸附在 NPs 表面上的蛋白质的组成取决于形成蛋白质冠的温度。此外，NPs 的摄取也受到温度的影响。因此，温度是生物纳米相互作用定量研究中需要仔细控制的重要参数。
