Grup d'Enginyeria de Materials (GEMAT), Institut Quimic de Sarrià, Universitat Ramon Llull , Via Augusta 390, 08017 Barcelona, Spain.
ACS Nano. 2013 Nov 26;7(11):10066-74. doi: 10.1021/nn404166q. Epub 2013 Oct 15.
We manipulate the passive release rates of DNA payloads on protein coronas formed around nanoparticles (NPs) by varying the corona composition. The coronas are prepared using a mixture of hard and soft corona proteins. We form coronas around gold nanorods (NRs), nanobones (NBs), and carbon nanotubes (CNTs) from human serum (HS) and find that tuning the amount of human serum albumin (HSA) in the NR-coronas (NR-HS-DNA) changes the payload release profile. The effect of buffer strength, HS concentration, and concentration of the cetyltrimethylammonium bromide (CTAB) passivating the NP surfaces on passive release is explored. We find that corona properties play an important role in passive release, and concentrations of CTAB, HS, and phosphate buffer used in corona formation can tune payload release profiles. These advances in understanding protein corona properties bring us closer toward developing a set of basic design rules that enable their manipulation and optimization for particular biological applications.
我们通过改变蛋白冠组成来操纵纳米颗粒（NPs）周围形成的蛋白冠上 DNA 有效负载的被动释放率。我们使用硬蛋白和软蛋白冠蛋白的混合物来制备蛋白冠。我们从人血清（HS）中制备金纳米棒（NR）、纳米骨（NB）和碳纳米管（CNT）的蛋白冠，并发现调节 NR 冠中的人血清白蛋白（HSA）的量（NR-HS-DNA）会改变有效负载的释放曲线。我们还研究了缓冲液强度、HS 浓度以及包覆 NP 表面的十六烷基三甲基溴化铵（CTAB）的浓度对被动释放的影响。我们发现蛋白冠特性在被动释放中起着重要作用，并且在形成蛋白冠中使用的 CTAB、HS 和磷酸盐缓冲液的浓度可以调节有效负载释放曲线。对蛋白冠特性的这些深入理解使我们更接近于开发一套基本的设计规则，从而能够对其进行操纵和优化，以应用于特定的生物学应用。
