Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Pécs, Hungary; Department of Diagnostic Laboratory, János Balassa Hospital, County Tolna, Szekszárd, Hungary.
Clin Microbiol Infect. 2014 Jan;20(1):O27-9. doi: 10.1111/1469-0691.12293. Epub 2013 Jun 28.
Since November 2009 carbapenemase-producing Klebsiella pneumoniae isolates have been detected in increasing numbers at the Clinical Centre University of Pécs. Molecular typing was performed for 102 clinical isolates originating from different time periods and various departments of the Clinical Centre. Pulsed-field gel electrophoresis revealed the predominance of a single clone (101/102), identified as sequence type ST15. PCR and sequencing showed the presence of blaCTX-M-15 and blaVIM-4 genes. The blaVIM-4 was located on a class 1 integron designated In238b. To our knowledge, this is the first description of a blaVIM-4 gene in the predominant CTX-M-15 extended spectrum β-lactamase-producing Hungarian Epidemic Clone/ST15.
自 2009 年 11 月以来，佩奇大学临床中心发现越来越多的产碳青霉烯酶肺炎克雷伯菌分离株。对来自不同时期和临床中心不同科室的 102 株临床分离株进行了分子分型。脉冲场凝胶电泳显示，单一克隆（101/102）占主导地位，被鉴定为 ST15 序列型。PCR 和测序显示 blaCTX-M-15 和 blaVIM-4 基因的存在。blaVIM-4 位于 1 类整合子 In238b 上。据我们所知，这是在匈牙利流行 CTX-M-15 型产超广谱β-内酰胺酶的优势克隆/ST15 中首次描述 blaVIM-4 基因。
