School of Chemical Engineering and ‡School of Mechanical Engineering, Yeungnam University , Gyeongsan 712-749, Republic of Korea.
J Agric Food Chem. 2013 Jul 24;61(29):7120-6. doi: 10.1021/jf4009313. Epub 2013 Jul 16.
Pathogenic biofilms are associated with persistent infection due to their high resistances to diverse antibiotics. Pseudomonas aeruginosa infects plants, animals, and humans and is a major cause of nosocomial diseases in patients with cystic fibrosis. In the present study, the antibiofilm abilities of 522 plant extracts against P. aeruginosa PA14 were examined. Three Carex plant extracts at a concentration of 200 μg/mL inhibited P. aeruginosa biofilm formation by >80% without affecting planktonic cell growth. In the most active extract of Carex pumila , resveratrol dimer ε-viniferin was one of the main antibiofilm compounds against P. aeruginosa. Interestingly, ε-viniferin at 10 μg/mL inhibited biofilm formation of enterohemorrhagic Escherichia coli O157:H7 by 98%. Although Carex extracts and trans-resveratrol are known to possess antimicrobial activity, this study is the first to report that C. pumila extract and ε-viniferin have antibiofilm activity against P. aeruginosa and E. coli O157:H7.
致病生物膜因其对各种抗生素的高抵抗力而与持续性感染有关。铜绿假单胞菌感染植物、动物和人类，是囊性纤维化患者医院获得性疾病的主要病因。在本研究中，检测了 522 种植物提取物对铜绿假单胞菌 PA14 的抗生物膜能力。三种浓度为 200μg/ml 的薹草植物提取物对铜绿假单胞菌生物膜形成的抑制率>80%，而对浮游细胞生长没有影响。在最活跃的薹草提取物中，白藜芦醇二聚体 ε-viniferin 是针对铜绿假单胞菌的主要抗生物膜化合物之一。有趣的是，10μg/ml 的 ε-viniferin 可抑制肠出血性大肠杆菌 O157:H7 的生物膜形成 98%。虽然已知薹草提取物和反式白藜芦醇具有抗菌活性，但本研究首次报道了薹草提取物和 ε-viniferin 对铜绿假单胞菌和大肠杆菌 O157:H7 具有抗生物膜活性。
