CSIRO Plant Industry, GPO Box 1600, Canberra 2601, ACT, Australia.
BMC Plant Biol. 2013 Jul 2;13:96. doi: 10.1186/1471-2229-13-96.
Adult plant rust resistance genes Lr67 and Lr34 confer race non-specific resistance to multiple fungal pathogens of wheat. Induced, susceptible mutants were characterised for both genes.
Three categories of Lr34 mutants were identified that were either partial susceptible, fully susceptible or hyper-susceptible to stripe rust and leaf rust. The likely impact of the mutational change on the predicted Lr34 protein correlated with differences in response to rust infection. Four independent Lr67 mutants were recovered that were susceptible to stripe rust, leaf rust and stem rust pathogens, including one possible hyper-susceptible Lr67 mutant.
Detailed study of Lr34 mutants revealed that subtle changes in resistance response to multiple pathogens were correlated with mutational changes in the predicted protein. Recovery of independent Lr67 mutants indicates that as for Lr34, a single gene at the Lr67 locus is likely to confer resistance to multiple pathogens. The infection phenotypes of Lr67 mutants closely resembled that of Lr34 mutants.
成株期抗锈病基因 Lr67 和 Lr34 赋予小麦对多种真菌病原体的非专化性抗性。本研究对这两个基因的诱导敏感突变体进行了特征分析。
鉴定出三种 Lr34 突变体，它们对条锈病和叶锈病的敏感性分别为部分敏感、完全敏感或超敏感。突变对预测的 Lr34 蛋白的可能影响与对锈病感染的反应差异相关。恢复了四个独立的 Lr67 突变体，它们对条锈病、叶锈病和秆锈病病原体均敏感，包括一个可能的超敏感 Lr67 突变体。
对 Lr34 突变体的详细研究表明，对多种病原体的抗性反应的细微变化与预测蛋白中的突变变化相关。独立的 Lr67 突变体的恢复表明，与 Lr34 一样，Lr67 基因座上的单个基因可能赋予对多种病原体的抗性。Lr67 突变体的感染表型与 Lr34 突变体非常相似。
