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检测万古霉素敏感粪肠球菌中 vanC1 基因的转录。

Detection of vanC1 gene transcription in vancomycin-susceptible Enterococcus faecalis.

机构信息

Programa de Pós-Graduação em Microbiologia Agrícola e do Ambiente, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.

出版信息

Mem Inst Oswaldo Cruz. 2013 Jun;108(4):453-6. doi: 10.1590/S0074-0276108042013009.

DOI:10.1590/S0074-0276108042013009
PMID:23828012
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3970631/
Abstract

Here we report the presence and expression levels of the vanC1 and vanC(2/3) genes in vancomycin-susceptible strains of Enterococcus faecalis. The vanC1 and vanC(2/3) genes were located in the plasmid DNA and on the chromosome, respectively. Specific mRNA of the vanC1 gene was detected in one of these strains. Additionally, analysis of the vanC gene sequences showed that these genes are related to the vanC genes of Enterococcus gallinarum and Enterococcus casseliflavus. The presence of vanC genes is useful for the identification of E. gallinarum and E. casseliflavus. Moreover, this is the first report of vanC mRNA in E. faecalis.

摘要

在这里，我们报告了万古霉素敏感性粪肠球菌中 vanC1 和 vanC(2/3) 基因的存在和表达水平。vanC1 和 vanC(2/3) 基因分别位于质粒 DNA 和染色体上。在其中一个菌株中检测到了 vanC1 基因的特异性 mRNA。此外，对 vanC 基因序列的分析表明，这些基因与鹑鸡肠球菌和黄色肠球菌的 vanC 基因有关。vanC 基因的存在有助于鹑鸡肠球菌和黄色肠球菌的鉴定。此外，这是粪肠球菌中 vanC mRNA 的首次报道。

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