Department of Evolutionary and Environmental Biology, Faculty of Natural Sciences, University of Haifa, Mount Carmel, 31905 Haifa, Israel.
Department of Biology and Environment, Faculty of Natural Sciences, University of Haifa, Oranim, Tivon 36006, Israel.
Int J Syst Evol Microbiol. 2013 Nov;63(Pt 11):4259-4265. doi: 10.1099/ijs.0.052217-0. Epub 2013 Jul 5.
Gram-negative, rod-shaped, oxidase-negative, facultatively anaerobic, yellow-orange-pigmented and motile bacterial strains, designated 8N4(T), 9N2 and 10N3, were isolated from flower nectar of Amygdalus communis (almond) and Citrus paradisi (grapefruit). The 16S rRNA gene sequences of the strains shared highest sequence similarity of 97.0 % with that of Phaseolibacter flectens ATCC 12775(T) and lower similarity with sequences from other type strains of genera of the Enterobacteriaceae. A polyphasic approach that included determination of phenotypic properties and phylogenetic analysis based on 16S rRNA, gyrB, rpoB and atpD gene sequences supported the classification of strains 8N4(T), 9N2 and 10N3 within a novel species in a novel genus in the family Enterobacteriaceae. Strain 8N4(T), and the reference strains of the novel species, grew at 4-35 °C (optimum, 28-30 °C), with 0-5.0 % NaCl (optimum, 3 % NaCl) and with 0-60 % sucrose (optimum, 10-25 % sucrose). Their major cellular fatty acids were C16 : 0, C17 : 0 cyclo, C18 : 1ω7c and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH). The DNA G+C content of strain 8N4(T) was 46.8 mol%. On the basis of phenotypic properties and phylogenetic distinctiveness, the floral nectar isolates are classified within a novel species in a new genus in the family Enterobacteriaceae, for which the name Rosenbergiella nectarea gen. nov., sp. nov. is proposed. The type strain of Rosenbergiella nectarea is 8N4(T) ( = LMG 26121(T) = DSM 24150(T)).
革兰氏阴性、杆状、氧化酶阴性、兼性厌氧、呈黄色橙色、运动的细菌菌株，命名为 8N4(T)、9N2 和 10N3，从扁桃（杏仁）和葡萄柚（葡萄柚）的花蜜中分离出来。这些菌株的 16S rRNA 基因序列与嗜纤维菌属的 Phaseolibacter flectens ATCC 12775(T)的序列相似度最高，为 97.0%，与其他肠杆菌科属的模式菌株的序列相似度较低。一种多相方法，包括表型特征的测定和基于 16S rRNA、gyrB、rpoB 和 atpD 基因序列的系统发育分析，支持菌株 8N4(T)、9N2 和 10N3 分类为肠杆菌科一个新属中的一个新种。菌株 8N4(T)和新种的参考菌株在 4-35°C 下生长（最佳温度为 28-30°C），0-5.0%NaCl（最佳为 3%NaCl）和 0-60%蔗糖（最佳为 10-25%蔗糖）。它们的主要细胞脂肪酸为 C16:0、C17:0 环、C18:1ω7c 和总和特征 3（C16:1ω7c 和/或 iso-C15:0 2-OH）。菌株 8N4(T)的 DNA G+C 含量为 46.8mol%。基于表型特征和系统发育的独特性，这些花的花蜜分离物被分类为肠杆菌科一个新属中的一个新种，命名为 Rosenbergiella nectarea 属，种，新种。Rosenbergiella nectarea 的模式菌株为 8N4(T)（=LMG 26121(T)=DSM 24150(T)）。
