丝状植物病毒在健康小鼠和荷瘤小鼠体内的生物分布与清除
Biodistribution and clearance of a filamentous plant virus in healthy and tumor-bearing mice.
作者信息
Shukla Sourabh, Wen Amy M, Ayat Nadia R, Commandeur Ulrich, Gopalkrishnan Ramamurthy, Broome Ann-Marie, Lozada Kristen W, Keri Ruth A, Steinmetz Nicole F
机构信息
Department of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.
出版信息
Nanomedicine (Lond). 2014 Feb;9(2):221-35. doi: 10.2217/nnm.13.100. Epub 2013 Jul 9.
AIM
Nanoparticles based on plant viruses are emerging biomaterials for medical applications such as drug delivery and imaging. Their regular structures can undergo genetic and chemical modifications to carry large payloads of cargos, as well as targeting ligands. Of several such platforms under development, only few have been characterized in vivo. We recently introduced the filamentous plant virus, potato virus X (PVX), as a new platform. PVX presents with a unique nanoarchitecture and is difficult to synthesize chemically.
METHODS
Here, we present a detailed analysis of PVX biodistribution and clearance in healthy mice and mouse tumor xenograft models using a combination of ex vivo whole-organ imaging, quantitative fluorescence assays and immunofluorescence microscopy.
RESULTS & CONCLUSION: While up to 30% of the PVX signal was from the colon, mammary and brain tumor tissues, remaining particles were cleared by the reticuloendothelial system organs (the spleen and liver), followed by slower processing and clearance through the kidneys and bile.
目的
基于植物病毒的纳米颗粒正成为用于药物递送和成像等医学应用的新兴生物材料。它们的规则结构可进行基因和化学修饰，以携带大量货物以及靶向配体。在几个正在开发的此类平台中，只有少数在体内得到了表征。我们最近引入了丝状植物病毒马铃薯X病毒（PVX）作为一个新平台。PVX具有独特的纳米结构，且难以化学合成。
方法
在此，我们结合体外全器官成像、定量荧光测定和免疫荧光显微镜，对健康小鼠和小鼠肿瘤异种移植模型中PVX的生物分布和清除进行了详细分析。
结果与结论
虽然高达30%的PVX信号来自结肠、乳腺和脑肿瘤组织，但其余颗粒被网状内皮系统器官（脾脏和肝脏）清除，随后通过肾脏和胆汁进行较慢的处理和清除。
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