Rahul Suryawanshi, Chandrashekhar Patil, Hemant Borase, Chandrakant Narkhede, Laxmikant Shinde, Satish Patil
a School of Life Sciences, North Maharashtra University , Jalgaon , Maharashtra 425001 , India.
Nat Prod Res. 2014;28(17):1399-404. doi: 10.1080/14786419.2014.904310. Epub 2014 Apr 4.
Ineffectiveness of available nematicides and the high damage caused by plant-parasitic nematodes result in the urgent need to find some natural remedy for their control. Bioactivity of the pigment extracted from Serratia marcescens was screened for controlling nematodes at their juvenile stage. Test pigment was found effective against juvenile stages of Radopholus similis and Meloidogyne javanica at low concentrations (LC50 values, 83 and 79 μg/mL, respectively) as compared with positive control of copper sulphate (LC50 values, 380 and 280 μg/mL, respectively). The pigment also exhibited inhibition on nematode egg-hatching ability. Characterisation of extracted pigment with TLC, FTIR, HPLC, HPTLC and spectroscopic analysis confirmed the presence of prodigiosin as a bioactive metabolite. Considering the sensory mechanism of pathogen recognition by nematodes, the use of microbial secondary metabolites can be effective for nematode control rather than using whole organism.
现有杀线虫剂效果不佳以及植物寄生线虫造成的严重危害，使得迫切需要找到一些天然的防治方法。对从粘质沙雷氏菌中提取的色素的生物活性进行了筛选，以控制线虫的幼虫阶段。与硫酸铜阳性对照（LC50值分别为380和280μg/mL）相比，测试色素在低浓度下（LC50值分别为83和79μg/mL）对相似穿孔线虫和爪哇根结线虫的幼虫阶段有效。该色素对线虫卵孵化能力也有抑制作用。通过薄层色谱法、傅里叶变换红外光谱法、高效液相色谱法、高效薄层色谱法和光谱分析对提取的色素进行表征，证实了灵菌红素作为一种生物活性代谢物的存在。考虑到线虫识别病原体的传感机制，使用微生物次生代谢产物对线虫防治可能比对使用整个生物体更有效。
