IEEE Trans Biomed Circuits Syst. 2009 Apr;3(2):97-107. doi: 10.1109/TBCAS.2008.2006494.
We describe the design, fabrication, and performance of a class of simple handheld fluorometers. The devices consist of a sensor along with an integrated optical filter packaged in a handheld format. The sensor is a differential active pixel sensor with in-pixel correlated double sampling fabricated in a 0.5-mu m 2-poly 3-metal complementary metal-oxide semiconductor process and has a readout noise of 175.3 muV, reset noise of 360 muV, dynamic range of 59 dB, and conversion gain of 530 nV/e(-) . The filter is a high rejection chromophore embedded in a polymer film which is cast onto the chip. We show the results of bioassays utilizing two different single color fluorometers constructed by using the chromophores 2-(2'-hydroxy 5'-methylphenyl) benzotriazole and Sudan II with long-pass wavelengths of 400 nm and 540 nm, respectively. The bioassays measures metabolic activity and viability of biological cells, which are useful for cytotoxicity and pathogen detection applications.
我们描述了一类简单手持式荧光计的设计、制作和性能。这些设备由传感器和集成光学滤波器组成，封装在手持式格式中。传感器是一种具有像素内相关双采样的差分有源像素传感器，采用 0.5 微米 2 多晶硅 3 金属互补金属氧化物半导体工艺制造，读出噪声为 175.3 微伏，复位噪声为 360 微伏，动态范围为 59 分贝，转换增益为 530 纳伏/电子(-)。滤波器是一种嵌入聚合物薄膜中的高反射色素，该薄膜被浇铸到芯片上。我们展示了利用两种不同的单色彩光荧光计进行生物测定的结果，这两种荧光计分别使用长波为 400nm 和 540nm 的色素 2-(2'-羟基-5'-甲基苯基)苯并三唑和苏丹 II 构建。生物测定用于测量生物细胞的代谢活性和活力，这对于细胞毒性和病原体检测应用非常有用。
