Martinez-Martinez R E, Loyola-Rodriguez J P, Bonilla-Garro S E, Patiño-Marin N, Haubek D, Amano A, Poulsen K
San Luis Potosi University, Mexico.
J Clin Pediatr Dent. 2013 Spring;37(3):289-95. doi: 10.17796/jcpd.37.3.d70710016518p58n.
The aim of this study was to characterize the main periodontal bacterial species in Down syndrome (DS) patients with and without periodontitis.
This cross-sectional study involved 75 DS patients, 45 with and 30 without periodontitis. Informed consent, health and dental questionnaires and periodontitis diagnosis were performed PCR and LAMP assays were performed on subgingival dental plaque sample.
Tannerella forsythia was the most frequent bacteria detected in the group with and without periodontitis (95.5 and 63.3%) followed by Treponema denticola (88.8 and 50%) and Porphyromonas gingivalis (53.3 and 25% respectively). There were statistical differences between groups (p < 0.05). Pg fimA type I was the most frequent Porphyromonas gingivalis genotype. Two different sets of primers (Aa-F/Aa-R and ltx3/ltx4) were used to detect Aggregatibacter actinomycetemcomitans and different frequencies were obtained, (68% and 14.6% respectively), they had a weak correlation (Cohen Kappa = 0.16). After sequencing of PCR products, ltx3/ltx4 showed more specificity. JP2 clone of A. actinomycetemcomitans was not detected in any sample.
The composition of oral biofilm is fundamental for the development of periodontal disease independently of immunological alterations associated with DS. The frequency of detection of A. actinomycetemcomitans reported in the literature has a wide range, because the primers and probes applied
本研究的目的是对患有和未患牙周炎的唐氏综合征（DS）患者的主要牙周细菌种类进行特征分析。
这项横断面研究纳入了75名DS患者，其中45名患有牙周炎，30名未患牙周炎。获取知情同意书，进行健康和牙科问卷调查以及牙周炎诊断，并对龈下牙菌斑样本进行聚合酶链反应（PCR）和环介导等温扩增（LAMP）检测。
在患有和未患牙周炎的两组中，福赛坦纳菌是检测到的最常见细菌（分别为95.5%和63.3%），其次是具核梭杆菌（分别为88.8%和50%）和牙龈卟啉单胞菌（分别为53.3%和25%）。两组之间存在统计学差异（p<0.05）。牙龈卟啉单胞菌fimA I型是最常见的基因型。使用两组不同的引物（Aa - F/Aa - R和ltx3/ltx4）检测伴放线聚集杆菌，获得了不同的频率（分别为68%和14.6%），它们之间的相关性较弱（科恩卡方系数=0.16）。对PCR产物进行测序后，ltx3/ltx4显示出更高的特异性。在任何样本中均未检测到伴放线聚集杆菌的JP2克隆。
口腔生物膜的组成对于牙周疾病的发展至关重要，与DS相关的免疫改变无关。文献报道的伴放线聚集杆菌的检测频率范围很广，这是因为所应用的引物和探针不同。
