Kaplanoglu Gulnur T, Bahcelioglu Meltem, Gozil Rabet, Helvacioglu Fatma, Buru Ece, Tekindal Mustafa A, Erdogan Deniz, Calguner Engin
Department of Histology and Embryology, Faculty of Medicine, Gazi University, Besevler 06500, Ankara, Turkey.
Saudi Med J. 2013 Jul;34(7):734-43.
To investigate the possible therapeutic or protective effects of green tea in diabetic rat's testicular tissue, either as a single agent, or together with vitamin E.
The present study was carried out at the Faculty of Medicine, Gazi University, Ankara, Turkey from May to August 2011 for 10 weeks. Forty-eight adult male Wistar albino rats, weighting 250-300 g, were divided into 8 groups: control; nondiabetic vitamin E (0.4 mg/kg/NG); nondiabetic green tea (300 mg/kg/NG); nondiabetic vitamin E plus green tea administered groups; diabetic group (60 mg/kg/IV streptozotocin); diabetic vitamin E; diabetic green tea; and diabetic vitamin E plus green tea administered groups. Proliferative and apoptotic indexes were determined using anti-PCNA antibody immunohistochemistry and TUNEL assays respectively. Tubule degeneration was evaluated using the Johnson's score and also seminiferous tubules diameters, epithelial thickness were measured.
Histopathological examination in diabetic group revealed degenerative changes in the seminiferous tubules together with a statistically significant decrease in PCNA positive cells, in epithelial thickness, diameter of the tubules and in Johnson's score, while exhibited an increase in the number of apoptotic cells. When all these findings are considered together, the most successful protective effects in diabetes were obtained in the combined antioxidant group.
Combined therapy of vitamin E and green tea in diabetes was more effective than monotherapy. Therefore, these antioxidants may be use as a supporting therapy for reproductive dysfunction.
研究绿茶单独或与维生素E联合使用时，对糖尿病大鼠睾丸组织可能产生的治疗或保护作用。
本研究于2011年5月至8月在土耳其安卡拉加齐大学医学院进行，为期10周。将48只体重250 - 300克的成年雄性Wistar白化大鼠分为8组：对照组；非糖尿病维生素E组（0.4毫克/千克/非糖尿病组）；非糖尿病绿茶组（300毫克/千克/非糖尿病组）；非糖尿病维生素E加绿茶给药组；糖尿病组（60毫克/千克链脲佐菌素静脉注射）；糖尿病维生素E组；糖尿病绿茶组；糖尿病维生素E加绿茶给药组。分别使用抗增殖细胞核抗原（PCNA）抗体免疫组织化学和末端脱氧核苷酸转移酶介导的缺口末端标记（TUNEL）法测定增殖指数和凋亡指数。使用约翰逊评分评估肾小管变性，并测量生精小管直径和上皮厚度。
糖尿病组的组织病理学检查显示生精小管有退行性变化，同时PCNA阳性细胞数量、上皮厚度、小管直径和约翰逊评分在统计学上显著降低，而凋亡细胞数量增加。综合考虑所有这些发现，联合抗氧化剂组在糖尿病中获得了最成功的保护作用。
维生素E和绿茶联合治疗糖尿病比单一治疗更有效。因此，这些抗氧化剂可作为生殖功能障碍的辅助治疗方法。
