RIKEN Advanced Science Institute, Wako, Saitama, Japan.
Phys Chem Chem Phys. 2013 Sep 7;15(33):13713-22. doi: 10.1039/c3cp51806j. Epub 2013 Jul 17.
Metal nanoparticles have recently emerged as ubiquitous surface-enhanced Raman scattering (SERS) agents for nano-imaging and nano-analysis. These applications make use of the unique optical properties of metal nanoparticles to enhance the efficiency of Raman scattering, whereas the small size of the nanoparticles localizes the enhanced Raman scattering at the nanoscale. In this perspective, we review the recent progress in SERS nano-imaging and nano-spectroscopy using metal nanoparticles applied especially to biological samples and nanomaterials. For biosamples, the highly sensitive molecular SERS detection capability of metal nanoparticles is used to analyze intracellular molecules. For analysis of nanomaterials, an innovative approach called tip-enhanced Raman scattering (TERS) microscopy, where metal nanoparticles are used with an AFM cantilever, has proven to be extremely powerful for nano-imaging. The precise control and analysis of the interaction between the metal and the sample are thus the key to overcome the limitations of current microscopic and spectroscopic techniques, and to guide future developments.
金属纳米粒子最近作为普遍存在的表面增强拉曼散射（SERS）试剂在纳米成像和纳米分析中崭露头角。这些应用利用金属纳米粒子的独特光学性质来提高拉曼散射的效率，而纳米粒子的小尺寸将增强的拉曼散射定位在纳米尺度上。在这篇观点文章中，我们综述了使用金属纳米粒子进行 SERS 纳米成像和纳米光谱学的最新进展，特别是应用于生物样品和纳米材料的进展。对于生物样本，金属纳米粒子的高灵敏度分子 SERS 检测能力用于分析细胞内分子。对于纳米材料的分析，一种称为尖端增强拉曼散射（TERS）显微镜的创新方法已被证明非常强大，其中使用原子力显微镜（AFM）悬臂将金属纳米粒子与 AFM 悬臂结合使用。因此，精确控制和分析金属与样品之间的相互作用是克服当前微观和光谱技术限制并指导未来发展的关键。
