Yavari Hamid Reza, Shahi Shahriar, Rahimi Saeed, Shakouie Sahar, Roshangar Leila, Mesgari Abassi Mehran, Sattari Khavas Sahar
Department of Endodontics, Dental School, Tabriz University of Medical Sciences, Tabriz/ Iranian Center for Endodontic Research, Tehran, Iran.
Iran Endod J. 2009 Winter;4(1):25-30. Epub 2009 Jan 7.
The purpose of this study was to compare the histocompatibility of white (WMTA) and gray (GMTA) mineral trioxide aggregate mixed with 0.12% chlorhexidine (CHX) and distilled water (DW) in subcutaneous connective tissues of rats.
The freshly mixed WMTA and GMTA with CHX or DW were inserted in polyethylene tubes and implanted into dorsal subcutaneous connective tissue of 50 Wistar Albino rats; tissue biopsies were collected and were then examined histologically 7, 15, 30, 60 and 90 days after the implantation procedure. The histology results were scored from 1-4; score 4 was considered as the worst finding. Data were analyzed using one-way ANOVA tests.
All experimented materials were tolerated well by the connective tissues after 90-day evaluation, except for the WMTA/CHX group that had significantly more mean inflammatory scores (P<0.001). There was a statistically significant difference in the mean inflammation grades between experimental groups in each interval (P<0.001). After 90 days, GMTA/CHX group had the lowest inflammatory score.
Although adding CHX to WMTA produces significantly higher inflammatory response, it seems a suitable substitute for DW in combination with GMTA. Further research is necessary to recommend this mixture for clinical use.
本研究的目的是比较白色（WMTA）和灰色（GMTA）三氧化矿物凝聚体与0.12%洗必泰（CHX）和蒸馏水（DW）混合后在大鼠皮下结缔组织中的组织相容性。
将新鲜混合的WMTA和GMTA与CHX或DW装入聚乙烯管中，植入50只Wistar白化大鼠的背部皮下结缔组织；在植入手术后7、15、30、60和90天收集组织活检样本，然后进行组织学检查。组织学结果按1-4分进行评分；4分被认为是最差的结果。使用单向方差分析测试对数据进行分析。
90天评估后，除WMTA/CHX组平均炎症评分显著更高（P<0.001）外，所有实验材料在结缔组织中耐受性良好。各时间间隔实验组之间的平均炎症分级存在统计学显著差异（P<0.001）。90天后，GMTA/CHX组炎症评分最低。
虽然向WMTA中添加CHX会产生显著更高的炎症反应，但它似乎是与GMTA结合使用时DW的合适替代品。需要进一步研究以推荐这种混合物用于临床。
