Fayer R, Guidry A, Blagburn B L
Livestock and Poultry Sciences Institute, U.S. Department of Agriculture, Beltsville, Maryland 20705.
Infect Immun. 1990 Sep;58(9):2962-5. doi: 10.1128/iai.58.9.2962-2965.1990.
Infection with Cryptosporidium parvum, a ubiquitous protozoan parasite of virtually all mammals, can cause mild to severe diarrhea in immunocompetent hosts and life-threatening diarrhea in immunocompromised hosts. Passive immunotherapy of experimentally infected animals and naturally infected humans with hyperimmune bovine colostrum has been reported to be efficacious, whereas chemotherapy has not. In this study, the efficacy of specific immunoglobulin isotypes purified from bovine colostrum from a cow hyperimmunized with Cryptosporidium parvum was assessed in neonatal BALB/c mice. Mice were orally infected with oocysts and treated with whole whey immunoglobulin G1 (IgG1), IgG2, IgA, or IgM at six intervals from 22 to 66 h postinfection. In histologic sections of intestine examined at 72 h postinfection, the reduction in number of intestinal stages in treated mice versus untreated controls was very highly significant (P less than 0.0001). The greatest reduction in parasite number was found in mice treated with IgG1, IgA, or whey.
微小隐孢子虫是几乎所有哺乳动物中普遍存在的一种原生动物寄生虫，它可在免疫功能正常的宿主中引起轻度至重度腹泻，在免疫功能低下的宿主中引起危及生命的腹泻。据报道，用超免疫牛初乳对实验感染的动物和自然感染的人类进行被动免疫治疗是有效的，而化疗则不然。在本研究中，评估了从用微小隐孢子虫超免疫的奶牛的初乳中纯化的特异性免疫球蛋白同种型对新生BALB/c小鼠的疗效。小鼠经口感染卵囊，并在感染后22至66小时的六个时间间隔用全乳清免疫球蛋白G1（IgG1）、IgG2、IgA或IgM进行治疗。在感染后72小时检查的肠道组织切片中，治疗小鼠与未治疗对照相比，肠道阶段数量的减少非常显著（P小于0.0001）。在用IgG1、IgA或乳清治疗的小鼠中发现寄生虫数量减少最多。
