Taylor H R, Maclean I W, Brunham R C, Pal S, Whittum-Hudson J
Dana Center for Preventive Ophthalmology, Johns Hopkins University, Baltimore, Maryland 21205.
Infect Immun. 1990 Sep;58(9):3061-3. doi: 10.1128/iai.58.9.3061-3063.1990.
Two chlamydial proteins (HSP-60 and HSP-70) have marked homology with bacterial and mammalian heat shock proteins. Previous studies have indicated that when inoculated into the eyes of immune animals, a Triton X-100 extract of chlamydia containing HSP-60 induces an ocular delayed-type hypersensitivity reaction. The potential for HSP-70 to induce a similar reaction was tested in six cynomolgus monkeys that had been sensitized to both antigens by previous ocular chlamydial infection. Whereas the chlamydial extract containing HSP-60 induced a marked clinical response within 24 h of inoculation, no response followed inoculation of HSP-70 in the contralateral eye. The lack of a response to HSP-70 suggests that further assessment of its potential as a trachoma vaccine is warranted.
两种衣原体蛋白（热休克蛋白60和热休克蛋白70）与细菌和哺乳动物的热休克蛋白具有显著的同源性。先前的研究表明，当将含有热休克蛋白60的衣原体Triton X-100提取物接种到免疫动物的眼中时，会引发眼部迟发型超敏反应。在六只先前因眼部衣原体感染而对这两种抗原均致敏的食蟹猴中测试了热休克蛋白70诱导类似反应的可能性。含有热休克蛋白60的衣原体提取物在接种后24小时内引发了明显的临床反应，而在对侧眼接种热休克蛋白70后则无反应。对热休克蛋白70无反应表明有必要进一步评估其作为沙眼疫苗的潜力。
