Meng D, Francis L, Thompson I D, Mierke C, Huebner H, Amtmann A, Roy I, Boccaccini A R
Department of Materials, Imperial College London, London, SW7 2BP, UK.
J Mater Sci Mater Med. 2013 Dec;24(12):2809-17. doi: 10.1007/s10856-013-5012-4. Epub 2013 Jul 28.
Bioglass(®)-based scaffolds for bone tissue engineering have been developed, which can also serve as carriers for drug delivery. For this, P(3HB) microspheres (PMSs) loaded with tetracycline were fabricated and immobilised on the scaffold surfaces by a modified slurry dipping technique. The sustained drug delivery ability in simulated body fluid was confirmed by using UV-Vis absorption spectroscopy measurements. The MTT assay using mouse fibroblast cells provided evidence that the tetracycline loaded microspheres produced in this study show limited cytotoxicity. The scaffolds developed in this work provide mechanical support, adequate 3D surface roughness, bioactivity and controlled drug delivery function, and are thus interesting candidates for bone tissue engineering applications.
已经开发出用于骨组织工程的基于生物玻璃（®）的支架，其也可作为药物递送的载体。为此，制备了负载四环素的聚（3-羟基丁酸酯）微球（PMS），并通过改进的浆料浸渍技术将其固定在支架表面。通过紫外可见吸收光谱测量证实了在模拟体液中的持续药物递送能力。使用小鼠成纤维细胞的MTT试验提供了证据，表明本研究中制备的负载四环素的微球显示出有限的细胞毒性。这项工作中开发的支架提供了机械支撑、足够的三维表面粗糙度、生物活性和可控的药物递送功能，因此是骨组织工程应用中有趣的候选材料。
