Department of Materials, Imperial College London, Prince Consort Road, London, SW7 2BP, United Kingdom.
J Mater Sci Mater Med. 2011 Mar;22(3):557-69. doi: 10.1007/s10856-011-4240-8. Epub 2011 Feb 4.
There is increasing interest in developing scaffolds with therapeutic and antibacterial potential for bone tissue engineering. Silver is a proven antibacterial agent which bacteria such as MRSA have little or no defense against. Using an ion exchange method, silver ions have been introduced into 45S5 Bioglass(®) based scaffolds that were fabricated using the foam replication technique. This technique allows the introduction of Ag(+) ions onto the surface of the scaffold without compromising the scaffold bioactivity and other physical properties such as porosity. Controlling the amount of Ag(+) ions introduced onto the surface of the scaffold was achieved by tailoring the ion exchange parameters to fabricate samples with repeatable and predictable Ag(+) ion release behavior. In vitro studies in simulated body fluid were carried out to ensure that the scaffolds maintained their bioactivity after the introduction of Ag(+) ions. It was also shown that the addition of low concentrations (2000:1 w/w) of silver ions supported the attachment and viability of human periodontal ligament stromal cells on the 3D scaffolds. This work has thus confirmed ion exchange as an effective technique to introduce Ag(+) ions into 45S5 Bioglass(®) scaffolds without compromising the basic properties of 45S5 Bioglass(®) which are required for applications in bone tissue engineering.
人们越来越感兴趣的是开发具有治疗和抗菌潜力的支架用于骨组织工程。银是一种已被证实的抗菌剂，而耐甲氧西林金黄色葡萄球菌（MRSA）等细菌对此几乎没有或没有防御能力。通过离子交换方法，已经将银离子引入到 45S5 生物玻璃（®）基支架中，这些支架是使用泡沫复制技术制造的。该技术允许将 Ag(+)离子引入到支架的表面上，而不会损害支架的生物活性和其他物理性质，如孔隙率。通过调整离子交换参数来控制引入到支架表面的 Ag(+)离子的量，可以制造出具有可重复和可预测的 Ag(+)离子释放行为的样品。在模拟体液中的体外研究表明，在引入 Ag(+)离子后，支架仍能保持其生物活性。此外，还表明添加低浓度（2000:1 w/w）的银离子支持人牙周膜基质细胞在 3D 支架上的附着和存活。因此，这项工作证实了离子交换是一种将 Ag(+)离子引入 45S5 生物玻璃（®）支架而不损害 45S5 生物玻璃（®）基本性质的有效技术，这些性质是骨组织工程应用所必需的。
