Surfactant and Colloid Group, Department of Chemistry, The University of Hull, Hull, HU6 7RX, UK.
Nanoscale. 2013 Sep 21;5(18):8560-8. doi: 10.1039/c3nr01893h.
We have engineered a class of colloids which can recognize the shape and size of targeted microbial cells and selectively bind to their surfaces. These imprinted colloid particles, which we called "colloid antibodies", were fabricated by partial fragmentation of silica shells obtained by templating the targeted microbial cells. We successfully demonstrated the shape and size recognition between such colloidal imprints and matching microbial cells. High percentage of binding events of colloidal imprints with the size matching target particles was achieved. We demonstrated selective binding of colloidal imprints to target microbial cells in a binary mixture of cells of different shapes and sizes, which also resulted in high binding selectivity. We explored the role of the electrostatic interactions between the target cells and their colloid imprints by pre-coating both of them with polyelectrolytes. Selective binding occurred predominantly in the case of opposite surface charges of the colloid cell imprint and the targeted cells. The mechanism of the recognition is based on the amplification of the surface adhesion in the case of shape and size match due to the increased contact area between the target cell and the colloidal imprint. We also tested the selective binding for colloid imprints of particles of fixed shape and varying sizes. The concept of cell recognition by colloid imprints could be used for development of colloid antibodies for shape-selective binding of microbes. Such colloid antibodies could be additionally functionalized with surface groups to enhance their binding efficiency to cells of specific shape and deliver a drug payload directly to their surface or allow them to be manipulated using external fields. They could benefit the pharmaceutical industry in developing selective antimicrobial therapies and formulations.
我们设计了一类胶体，它可以识别目标微生物细胞的形状和大小，并选择性地与其表面结合。这些被我们称为“胶体抗体”的印迹胶体颗粒是通过模板化靶向微生物细胞来部分破碎获得的二氧化硅壳得到的。我们成功地证明了胶体印迹与匹配微生物细胞之间的形状和大小识别。胶体印迹与尺寸匹配的目标颗粒的结合事件的高百分比得到了实现。我们证明了胶体印迹在不同形状和大小的细胞的二元混合物中对目标微生物细胞的选择性结合，这也导致了高结合选择性。我们通过用聚电解质预先涂覆两者来探索目标细胞与其胶体印迹之间的静电相互作用的作用。在胶体细胞印迹和目标细胞的表面电荷相反的情况下，主要发生选择性结合。识别的机制基于由于目标细胞和胶体印迹之间的接触面积增加，在形状和尺寸匹配的情况下表面粘附的放大。我们还测试了胶体印迹对固定形状和变化尺寸的颗粒的选择性结合。胶体印迹的细胞识别概念可用于开发用于微生物形状选择性结合的胶体抗体。这种胶体抗体可以用表面基团进一步功能化，以提高其与特定形状的细胞的结合效率，并直接将药物有效负载递送到其表面，或允许使用外部场来操纵它们。它们可以使制药行业受益于开发选择性抗菌疗法和制剂。
