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分子印迹技术用于检测食源性病原体细菌。

Molecular imprinting technology for sensing foodborne pathogenic bacteria.

机构信息

Department of Food Science and Agricultural Chemistry, Faculty of Agricultural and Environmental Sciences, McGill University, Sainte-Anne-de-Bellevue, Quebec, H9X 3V9, Canada.

出版信息

Anal Bioanal Chem. 2021 Jul;413(18):4581-4598. doi: 10.1007/s00216-020-03138-x. Epub 2021 Feb 9.

Abstract

Foodborne diseases caused by bacterial pathogens pose a widespread and growing threat to public health in the world. Rapid detection of pathogenic bacteria is of great importance to prevent foodborne diseases and ensure food safety. However, traditional detection methods are time-consuming, labour intensive and expensive. In recent years, many attempts have been made to develop alternative methods for bacterial detection. Biosensors integrated with molecular imprinted polymers (MIPs) and various transducer platforms are among the most promising candidates for the detection of pathogenic bacteria in a highly sensitive, selective and ultra-rapid manner. In this review, we summarize the most recent advances in molecular imprinting for bacterial detection, introduce the underlying recognition mechanisms and highlight the applications of MIP-based biosensors. In addition, the challenges and future perspectives are discussed with the aim of accelerating the development of MIP-based biosensors and extending their applications.

摘要

食源性疾病由细菌病原体引起,对世界公共卫生构成广泛且日益严重的威胁。快速检测致病菌对于预防食源性疾病和确保食品安全至关重要。然而,传统的检测方法既耗时、费力又昂贵。近年来,人们尝试了许多方法来开发替代的细菌检测方法。基于分子印迹聚合物(MIPs)和各种换能器平台的生物传感器是最有前途的候选者之一,可实现对致病菌的高灵敏度、选择性和超快速检测。在本文中,我们总结了分子印迹在细菌检测方面的最新进展,介绍了其基本识别机制,并重点介绍了基于 MIP 的生物传感器的应用。此外,还讨论了挑战和未来展望,旨在加速基于 MIP 的生物传感器的发展并拓展其应用。

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