Builders P F, Kabele-Toge B, Builders M, Chindo B A, Anwunobi Patricia A, Isimi Yetunde C
Department of Pharmaceutical Technology and Raw Material Development, National Institute for Pharmaceutical Research and Development, Abuja, Nigeria.
Indian J Pharm Sci. 2013 Jan;75(1):45-52. doi: 10.4103/0250-474X.113549.
Wound healing agents support the natural healing process, reduce trauma and likelihood of secondary infections and hasten wound closure. The wound healing activities of water in oil cream of the methanol extract of Hibiscus sabdariffa L. (Malvaceae) was evaluated in rats with superficial skin excision wounds. Antibacterial activities against Pseudomonas aeroginosa, Staphylococcus aureus and Echerichia coli were determined. The total flavonoid content, antioxidant properties and thin layer chromatographic fingerprints of the extract were also evaluated. The extract demonstrated antioxidant properties with a total flavonoid content of 12.30±0.09 mg/g. Six reproducible spots were obtained using methanol:water (95:5) as the mobile phase. The extract showed no antimicrobial activity on the selected microorganisms, which are known to infect and retard wound healing. Creams containing H. sabdariffa extract showed significant (P<0.05) and concentration dependent wound healing activities. There was also evidence of synergism with creams containing a combination of gentamicin and H. sabdariffa extract. This study, thus, provides evidence of the wound healing potentials of the formulated extract of the calyces of H. sabdariffa and synergism when co-formulated with gentamicin.
伤口愈合剂可支持自然愈合过程，减少创伤和继发感染的可能性，并加速伤口闭合。对玫瑰茄（锦葵科）甲醇提取物的油包水乳膏的伤口愈合活性在大鼠浅表皮肤切除伤口模型中进行了评估。测定了其对铜绿假单胞菌、金黄色葡萄球菌和大肠杆菌的抗菌活性。还评估了提取物的总黄酮含量、抗氧化性能和薄层色谱指纹图谱。提取物表现出抗氧化性能，总黄酮含量为12.30±0.09 mg/g。以甲醇:水（95:5）为流动相获得了6个可重现的斑点。提取物对所选已知会感染并阻碍伤口愈合的微生物没有抗菌活性。含玫瑰茄提取物的乳膏表现出显著（P<0.05）且浓度依赖性的伤口愈合活性。同时也有证据表明含庆大霉素和玫瑰茄提取物组合的乳膏具有协同作用。因此，本研究提供了玫瑰茄花萼配方提取物的伤口愈合潜力以及与庆大霉素共同配方时的协同作用的证据。
