Bian Qingya, Yang Hui, Chan Chi-On, Jin Dengping, Mok Daniel Kam-Wah, Chen Sibao
Yancheng Institute of Health Science; Yancheng 224005, China.
Chem Pharm Bull (Tokyo). 2013;61(8):802-8. doi: 10.1248/cpb.c12-01058.
Curculiginis Rhizoma (Curculigo orchioides GAERTN.) is a well-known Chinese herbal medicine, as well as an important Rasayana drug in India. Current criteria of quality control on this herb are to quantitatively analyze single compound curculigoside, which fail to comprehensively evaluate quality of this herb. In this paper, a simple and reliable HPLC coupled with diode array detector (DAD) method was developed to evaluate the quality of Curculiginis Rhizoma through establishing chromatographic fingerprint and simultaneously quantitating four phenolic compounds, orcinol glucoside, orcinol, 2,6-dimethoxybenzoic acid and curculigoside. The fingerprint displayed eleven common peaks, and the similarity index of different samples was in a range of 0.890-0.977. Validation of the method was acceptable, with 96.03-102.82% accuracy in recovery test and inter and intra-day precisions were less than 2%. This developed method by having a combination of chromatographic fingerprint and quantitation analysis could be applied to the quality control of Curculiginis Rhizoma.
仙茅是一种著名的中药材，也是印度一种重要的回春药。目前该药材的质量控制标准是对单一化合物仙茅苷进行定量分析，这无法全面评估该药材的质量。本文建立了一种简单可靠的高效液相色谱-二极管阵列检测器（HPLC-DAD）方法，通过建立色谱指纹图谱并同时定量测定四种酚类化合物（邻苯二酚葡萄糖苷、邻苯二酚、2,6-二甲氧基苯甲酸和仙茅苷）来评估仙茅的质量。指纹图谱显示有11个共有峰，不同样品的相似度指数在0.890-0.977之间。该方法验证结果良好，回收率试验准确度为96.03%-102.82%，日间和日内精密度均小于2%。这种将色谱指纹图谱与定量分析相结合的方法可用于仙茅的质量控制。
