Hubálek Zdenek, Ludvíková Eva, Jahn Petr, Treml František, Rudolf Ivo, Svobodová Petra, Šikutová Silvie, Betášová Lenka, Bíreš Jozef, Mojžíš Miroslav, Tinák Martin, Boldižár Martin, Citsoňová Gabriela, Staššíková Zuzana
1 Institute of Vertebrate Biology , v.v.i., Academy of Sciences, 60365 Brno, Czech Republic .
Vector Borne Zoonotic Dis. 2013 Oct;13(10):733-8. doi: 10.1089/vbz.2012.1159. Epub 2013 Aug 6.
A serological survey for West Nile virus (WNV) infection involved 395 horses from 43 administrative districts of the Czech Republic (163 animals) and 29 districts of Slovakia (232 animals), sampled between 2008 and 2011. Using a plaque-reduction neutralization microtest, antibodies to WNV were not detected in any horse from the Czech Republic, whereas 19 nonvaccinated horses from Slovakia had specific antibodies to WNV (no cross-reactions were observed with tick-borne encephalitis and Usutu flaviviruses in those animals). The seropositivity rate of nonvaccinated horses in Slovakia was 8.3% (95% confidence interval [CI] 4.7-11.9%), and autochthonous local infection with WNV occurred at least in 11, i.e., 4.8% (95% CI 2.0-7.6%) of the animals. All seropositive horses lived in six lowland districts of southern Slovakia; overall, 15.1% (95% CI 8.8-21.4%) of 126 nonvaccinated horses were seropositive in those districts, situated relatively closely to the border with Hungary, i.e., the country where WNV disease cases have been reported in birds, horses and humans since 2003.
一项针对西尼罗河病毒（WNV）感染的血清学调查涉及来自捷克共和国43个行政区的395匹马（163匹）和斯洛伐克29个行政区的232匹马，样本采集于2008年至2011年期间。使用蚀斑减少中和微量试验，在捷克共和国的任何马匹中均未检测到WNV抗体，而斯洛伐克的19匹未接种疫苗的马匹具有WNV特异性抗体（在这些动物中未观察到与蜱传脑炎病毒和乌苏图黄病毒的交叉反应）。斯洛伐克未接种疫苗马匹的血清阳性率为8.3%（95%置信区间[CI]4.7 - 11.9%），WNV的本地感染至少发生在11匹马中，即占动物总数的4.8%（95%CI 2.0 - 7.6%）。所有血清阳性马匹都生活在斯洛伐克南部的六个低地行政区；总体而言，在与匈牙利接壤且相对较近的那些行政区中，126匹未接种疫苗的马匹中有15.1%（95%CI 8.8 - 21.4%）血清呈阳性，自2003年以来，匈牙利已报告鸟类、马匹和人类感染WNV疾病的病例。
