Institute of Biomedicine, Pharmacology, PO Box 63, FIN-00014 University of Helsinki, Finland.
Valio Limited, R&D, PO Box 30, FIN-00039 Helsinki, Finland.
Br J Nutr. 2014 Jan 28;111(2):301-9. doi: 10.1017/S0007114513002420. Epub 2013 Aug 7.
The aims of the present study were to assess the possible differences in faecal microbiota between men with a low serum enterolactone concentration and those with a high concentration, and to investigate the impact of a synbiotic mixture on serum enterolactone concentration in men with a low concentration. We compared faecal microbiota between ten men with the lowest serum enterolactone concentration and ten men with the highest concentration at recruitment (n 84). Furthermore, we carried out a randomised, double-blind, placebo-controlled, cross-over intervention study (6-week intervention periods and 4-week washout period) to investigate the impact of a synbiotic mixture (two Lactobacillus strains, one Bifidobacterium strain, one Propionibacterium strain and galacto-oligosaccharides (32 g/l)) on serum enterolactone concentration in fifty-two men who had a concentration < 20 nmol/l. Serum sensitive C-reactive protein (CRP) concentration was measured at the end of the first intervention period. Men with a low serum enterolactone concentration when compared with those with a high concentration had less faecal bacteria, especially those belonging to the Lactobacillus-Enterococcus group (median 8·2 (interquartile range 7·8-8·4) log10 colony-forming units/g v. median 8·8 (interquartile range 8·5-8·9) log10 colony-forming units/g, P= 0·009). The synbiotic mixture that was used did not have a significant effect on serum enterolactone (synbiotic v. placebo ratio 0·96 (95 % CI 0·76, 1·22), P= 0·724) or serum sensitive CRP (synbiotic v. placebo ratio 0·99 (95 % CI 0·74, 1·33), P= 0·954) concentration. Men with a low serum enterolactone concentration harbour less colonic bacteria, especially those belonging to the Lactobacillus-Enterococcus group. A synbiotic mixture does not increase serum enterolactone concentration.
本研究的目的是评估血清肠内酯浓度低的男性与浓度高的男性之间粪便微生物群的可能差异，并研究共生混合物对低浓度男性血清肠内酯浓度的影响。我们在招募时比较了 10 名血清肠内酯浓度最低的男性和 10 名血清肠内酯浓度最高的男性的粪便微生物群（n=84）。此外，我们进行了一项随机、双盲、安慰剂对照、交叉干预研究（6 周干预期和 4 周洗脱期），以研究共生混合物（两种乳杆菌、一种双歧杆菌、一种丙酸杆菌和半乳糖寡糖（32g/l））对 52 名血清肠内酯浓度<20nmol/l 的男性血清肠内酯浓度的影响。在第一个干预期结束时测量血清敏感 C 反应蛋白（CRP）浓度。与血清肠内酯浓度高的男性相比，血清肠内酯浓度低的男性粪便细菌较少，尤其是属于乳杆菌-肠球菌组的细菌（中位数 8·2（四分位距 7·8-8·4）log10 菌落形成单位/g 与中位数 8·8（四分位距 8·5-8·9）log10 菌落形成单位/g，P=0·009）。所使用的共生混合物对血清肠内酯（共生物与安慰剂的比值为 0·96（95%置信区间为 0·76，1·22），P=0·724）或血清敏感 CRP（共生物与安慰剂的比值为 0·99（95%置信区间为 0·74，1·33），P=0·954）浓度没有显著影响。血清肠内酯浓度低的男性结肠细菌较少，尤其是属于乳杆菌-肠球菌组的细菌。共生混合物不能增加血清肠内酯浓度。
