Center for Natural Science and Humanities, Federal University of ABC (UFABC), Avenida dos Estados, 5001, Bloco B, Santo Andre, SP, Brazil.
Metallomics. 2013 Oct;5(10):1336-45. doi: 10.1039/c3mt00136a.
Highly complex analytical methods with different accuracies of measurement, reproducibilities and ease of analyses are currently being used to quantify metals in cellular media and tissue samples. In this review, the analytical methods commonly used for iron, copper and zinc quantification in mammalian cells are presented and discussed. Herein, we present a literature survey of the most commonly found concentrations of these metals in various mammalian cells in culture and tissues. The aim of this review is to help researchers in metallomic-related areas identify the method that best suits their needs for the accurate quantification of these metals in cells. This accuracy goes beyond simple knowledge of the limit of detection of each technique and needs to be evaluated through comparisons with similar previous studies.
目前，研究人员使用高度复杂的分析方法来测量细胞介质和组织样本中的金属，这些方法具有不同的测量精度、重现性和分析简便性。在本文中，我们对目前用于检测哺乳动物细胞中铁、铜和锌的分析方法进行了介绍和讨论。我们还对文献中培养的各种哺乳动物细胞和组织中这些金属的常见浓度进行了综述。本文旨在帮助从事金属组学相关研究的人员选择最合适的方法，以实现对细胞内这些金属的准确定量，这种准确性并不仅仅局限于检测技术的检出限，还需要通过与类似的先前研究进行比较来进行评估。
