Quadros N P, Roberts-Thomson P J, Gallus A S
Department of Haematology, Flinders Medical Centre, Bedford Park, S.A.
Rheumatol Int. 1990;10(3):113-9. doi: 10.1007/BF02274825.
A cellular enzyme-linked immunosorbent assay (ELISA) was used to detect circulating anti-endothelial cell antibodies (AECA) in sera of patients suffering from rheumatoid arthritis (RA). Felty's syndrome (FS), systemic lupus erythematosus (SLE), progressive systemic sclerosis (PSS) and those with a "lupus anticoagulant" (LA). IgG AECA were detected in RA, FS, SLE and LA sera, while IgM AECA were only detected in RA and FS. AECA were not specific for endothelial cells. However, IgG binding to endothelial cells and dermal fibroblasts was F(ab) mediated while in all other cell types tested, nonspecific binding most likely via the Fc region occurred. In RA and FS rheumatoid factor was shown to augment immunoglobulin binding to endothelial cells. Additional studies revealed that some of these pathological sera also contained cytotoxic IgG autoantibodies which fixed complement and damaged human umbilical vein endothelial cells in vitro. These studies suggest that a group of autoantibodies, present in a variety of collagen vascular disorders, react with endothelial cells and thus may be important aetiopathogenic factors in the vasculopathies associated with these disorders.
采用细胞酶联免疫吸附测定（ELISA）法检测类风湿关节炎（RA）患者血清中的循环抗内皮细胞抗体（AECA）。包括费尔蒂综合征（FS）、系统性红斑狼疮（SLE）、进行性系统性硬化症（PSS）以及患有“狼疮抗凝物”（LA）的患者。在RA、FS、SLE和LA患者血清中检测到IgG AECA，而IgM AECA仅在RA和FS中检测到。AECA并非内皮细胞所特有。然而，IgG与内皮细胞和真皮成纤维细胞的结合是由F（ab）介导的，而在所有其他检测的细胞类型中，最有可能是通过Fc区域发生非特异性结合。在RA和FS中，类风湿因子可增强免疫球蛋白与内皮细胞的结合。进一步研究表明，这些病理性血清中的一些还含有细胞毒性IgG自身抗体，它们可固定补体并在体外损伤人脐静脉内皮细胞。这些研究提示，存在于多种胶原血管疾病中的一组自身抗体可与内皮细胞发生反应，因此可能是与这些疾病相关的血管病变中的重要致病因素。
