Kelsey P R, Stevenson K J, Poller L
Thromb Haemost. 1984 Oct 31;52(2):172-5.
Liposomes of pure phospholipids were used in a modified APTT test system and the role of phosphatidyl serine (PS) in determining the sensitivity of the test system to the presence of lupus anticoagulants was assessed. Six consecutive patients with lupus anticoagulants and seven haemophiliacs with anticoagulants directed at specific coagulation factors, were studied. Increasing the concentration of phospholipid in the test system markedly reduced the sensitivity to lupus anticoagulants but had marginal effect on the specific factor inhibitors. The same effect was achieved when the content of PS alone was increased in a vehicle liposome of constant composition. The results suggest that the lupus anticoagulants can best be detected by a screening method using an APTT test with a reagent of low PS content. The use of a reagent rich in PS will largely abolish the lupus anticoagulant's effect on the APTT. An approach using the two different types of reagent may facilitate differentiation of lupus inhibitors from other types of anticoagulant.
纯磷脂脂质体用于改良的活化部分凝血活酶时间（APTT）检测系统，并评估了磷脂酰丝氨酸（PS）在确定该检测系统对狼疮抗凝物存在的敏感性方面的作用。研究了6例连续的狼疮抗凝物患者和7例针对特定凝血因子的抗凝血友病患者。增加检测系统中磷脂的浓度显著降低了对狼疮抗凝物的敏感性，但对特定因子抑制剂的影响很小。当在恒定组成的载体脂质体中单独增加PS的含量时，也能达到相同的效果。结果表明，使用低PS含量试剂的APTT检测筛查方法能够最好地检测出狼疮抗凝物。使用富含PS的试剂将在很大程度上消除狼疮抗凝物对APTT的影响。使用两种不同类型试剂的方法可能有助于区分狼疮抑制剂与其他类型的抗凝物。
