Department of Cell Biology and Genetics, Shenyang Medical College, Shenyang, Liaoning, PR China.
Gene. 2013 Oct 25;529(2):282-7. doi: 10.1016/j.gene.2013.07.033. Epub 2013 Aug 6.
The aim of this study was to estimate the allelic frequencies of the 19 STR loci with the Goldeneye™ DNA ID system 20A kit in a sample of 150 Manchu individuals from China to be used for forensic purposes and population studies. The observed heterozygosity(HO)values of these 19 STR loci ranged from 0.600 (D3S1358) to 0.914 (D18S51), the expected (HE) ranged from 0.615 (TPOX) to 0.876 (D16S1043). The power of discrimination (PD) values were found to range from 0.793 (TPOX) to 0.950 (D16S1043) and the probability of exclusion (PE) varies between 0.291 (D3S1358) and 0.825 (D18S51 and Penta E). Among all the 19 loci, D16S1043 had the highest polymorphism (PIC=0.860), whereas TPOX had the lowest (PIC=0.550). For the 19 loci, the combined power of discrimination and the combined probability of exclusion are 0.9999999999999999999942 and 0.999999996777, respectively. The phylogenetic tree established among worldwide population shows different populations who say the same language usually have a close genetic relationship with each other across the three language families studied (Sino-Tibetan, Altaic and Arabic).
本研究旨在估计 19 个 STR 基因座在来自中国的 150 名满族个体中的等位基因频率，该样本用于法医学和群体研究。这 19 个 STR 基因座的观察杂合度（HO）值范围从 0.600（D3S1358）到 0.914（D18S51），预期杂合度（HE）范围从 0.615（TPOX）到 0.876（D16S1043）。发现鉴别能力（PD）值范围从 0.793（TPOX）到 0.950（D16S1043），排除概率（PE）在 0.291（D3S1358）和 0.825（D18S51 和 Penta E）之间变化。在所有 19 个基因座中，D16S1043 的多态性最高（PIC=0.860），而 TPOX 的最低（PIC=0.550）。对于 19 个基因座，联合鉴别能力和联合排除概率分别为 0.9999999999999999999942 和 0.999999996777。在全球人群中建立的系统发育树表明，说同一种语言的不同人群通常在三个研究的语系（汉藏语系、阿尔泰语系和阿拉伯语系）中彼此之间具有密切的遗传关系。
