高效液相色谱法同时测定连钱草提取物中的四种生物活性化合物
Simultaneous determination of four bioactive compounds in Glechoma longituba extracts by high performance liquid chromatography.
作者信息
Shan Qiyuan, Cao Gang, Cai Hao, Cai Baochang
机构信息
Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, China ; Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, Hangzhou, P. R. China.
出版信息
Pharmacogn Mag. 2013 Jul;9(35):216-9. doi: 10.4103/0973-1296.113269.
BACKGROUND
Glechoma longituba, one of the long-practiced traditional Chinese medicines in history, is still commonly used nowadays in oriental countries. Previous study indicates that phenolic acid and flavonoids have considerable bioactivities, thus, chlorogenic acid, caffeic acid, apigenin-7-O-glucoside and rosmarinic acid were chosen as the marker components for the simultaneous determination to evaluate the intrinsic quality of G. longituba, and related high performance liquid chromatographic method was urgent to established.
MATERIALS AND METHODS
A HPLC method was established for simultaneous determination of four major active components in G. longituba, three batches which collected from different suppliers in Zhejiang, Anhui and Jiangsu Provinces were determined.
RESULTS
The contents of chlorogenic acid, caffeic acid, apigenin-7-O-glucoside and rosmarinic acid in G. longituba samples analyzed were 0.00225-0.00234%, 0.0238-0.0242%, 0.00271-0.00313%, 0.830-0.896%, respectively.
CONCLUSION
The developed method can be applied to the intrinsic quality control of G. longituba.
背景
连钱草是历史上长期应用的传统中药之一，至今仍在东方国家广泛使用。先前的研究表明酚酸和黄酮类化合物具有相当大的生物活性，因此，选择绿原酸、咖啡酸、芹菜素 -7-O-葡萄糖苷和迷迭香酸作为同时测定的指标成分，以评价连钱草的内在质量，迫切需要建立相关的高效液相色谱法。
材料与方法
建立了一种高效液相色谱法同时测定连钱草中四种主要活性成分的含量，并对从浙江、安徽和江苏三省不同供应商处采集的三批样品进行了测定。
结果
所分析的连钱草样品中绿原酸、咖啡酸、芹菜素 -7-O-葡萄糖苷和迷迭香酸的含量分别为0.00225 - 0.00234%、0.0238 - 0.0242%、0.00271 - 0.00313%、0.830 - 0.896%。
结论
所建立的方法可用于连钱草的内在质量控制。
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