Dam M, Ori C, Pizzolato G, Ricchieri G L, Pellegrini A, Giron G P, Battistin L
Department of Neurology, University of Padua, Italy.
Anesthesiology. 1990 Sep;73(3):499-505. doi: 10.1097/00000542-199009000-00021.
The autoradiographic 14C-2-deoxy-D-glucose method was used to determine local cerebral glucose utilization (LCGU) during propofol anesthesia and recovery in 52 regions of the rat brain. Control rats intravenously received 5 ml.kg-1.h-1 of the egg-oil-glycerol emulsion that constitutes the vehicle for propofol. Anesthetized animals received an iv bolus of propofol (20 mg/kg) followed by continuous infusion of the anesthetic at 12.5, 25, or 50 mg.kg-1.h-1 for 1 h prior to injection of 14C-2-deoxy-D-glucose and for the following 45 min. In addition, a fifth group of animals were studied immediately after awakening from a 20 mg/kg bolus of propofol as indicated by the first reappearance of head lift. All rats were spontaneously breathing room air throughout the experimental procedure. The general pattern of the cerebral metabolic response to propofol anesthesia was a dose-related, widespread depression of LCGU. At the three infusion rates of propofol tested, overall mean LCGU was reduced by 33%, 49%, and 55%, respectively, and significant decreases were observed in 60%, 85%, and 90% of the regions assayed. These effects were rapidly reversible, since in the recovery group, LCGU returned to near control values in the majority of the brain areas. Although all of the anatomofunctional systems (sensorimotor, extrapyramidal, limbic, and reticular) were involved, forebrain structures showed a greater sensitivity to the depressant action of propofol than did hindbrain regions.(ABSTRACT TRUNCATED AT 250 WORDS)
采用放射自显影14C-2-脱氧-D-葡萄糖法，测定了52个大鼠脑区在丙泊酚麻醉及苏醒过程中的局部脑葡萄糖利用率（LCGU）。对照组大鼠静脉输注5 ml·kg-1·h-1构成丙泊酚溶媒的卵油甘油乳剂。麻醉动物先静脉注射一剂丙泊酚（20 mg/kg），然后在注射14C-2-脱氧-D-葡萄糖前1小时及随后45分钟，分别以12.5、25或50 mg·kg-1·h-1的速度持续输注麻醉药。此外，第五组动物在静脉注射20 mg/kg丙泊酚后首次出现抬头动作表明苏醒时，立即进行研究。在整个实验过程中，所有大鼠均自主呼吸室内空气。丙泊酚麻醉引起的脑代谢反应总体模式是与剂量相关的、广泛的LCGU降低。在测试的三种丙泊酚输注速率下，总体平均LCGU分别降低了33%、49%和55%，在所检测的脑区中，分别有60%、85%和90%出现显著降低。这些作用迅速可逆，因为在恢复组中，大多数脑区的LCGU恢复到接近对照值。尽管所有解剖功能系统（感觉运动、锥体外系、边缘系统和网状系统）均受累，但前脑结构对丙泊酚的抑制作用比后脑区域更为敏感。（摘要截于250字）
