Instituto de Neurociencias, Consejo Superior de Investigaciones Científicas & Universidad Miguel Hernández, Sant Joan d'Alacant, Spain.
Nat Neurosci. 2013 Sep;16(9):1199-210. doi: 10.1038/nn.3485. Epub 2013 Aug 11.
In the cerebral cortex, pyramidal cells and interneurons are generated in distant germinal zones, and so the mechanisms that control their precise assembly into specific microcircuits remain an enigma. Here we report that cortical interneurons labeled at the clonal level do not distribute randomly but rather have a strong tendency to cluster in the mouse neocortex. This behavior is common to different classes of interneurons, independently of their origin. Interneuron clusters are typically contained within one or two adjacent cortical layers, are largely formed by isochronically generated neurons and populate specific layers, as revealed by unbiased hierarchical clustering methods. Our results suggest that different progenitor cells give rise to interneurons populating infra- and supragranular cortical layers, which challenges current views of cortical neurogenesis. Thus, specific lineages of cortical interneurons seem to be produced to primarily mirror the laminar structure of the cerebral cortex, rather than its columnar organization.
在大脑皮层中，锥体神经元和中间神经元在遥远的生发区产生，因此控制它们精确组装成特定微电路的机制仍然是一个谜。在这里，我们报告说，在克隆水平上标记的皮层中间神经元不会随机分布，而是有很强的聚类倾向。这种行为在不同类别的中间神经元中是共同的，与它们的起源无关。中间神经元簇通常包含在一个或两个相邻的皮层层内，主要由同期产生的神经元组成，并通过无偏聚类方法显示出在特定的皮层层中存在。我们的结果表明，不同的祖细胞产生了中间神经元，这些中间神经元填充了皮质的下颗粒和上颗粒层，这挑战了当前的皮质神经发生观点。因此，皮质中间神经元的特定谱系似乎主要是为了反映大脑皮层的层状结构，而不是其柱状组织。
