Tripathi Anurag, Riddell James, Chronis Nikos
Department of Mechanical Engineering, University of Michigan Ann Arbor, Michigan USA.
Sens Actuators B Chem. 2013 Sep 1;186:244-251. doi: 10.1016/j.snb.2013.05.095.
We present a microfluidic biochip for trapping single white blood cells (WBCs). The novel biochip, microfabricated using standard surface micromachining processes, consists of an array of precisely engineered microholes that confine single cells in a tight, three dimensional space and mechanically immobilize them. A high (> 87%) trapping efficiency was achieved when WBC-containing samples were delivered to the biochip at the optimal pressure of 3 psi. The biochip can efficiently trap up to 7,500 cells, maintaining a high trapping efficiency even when the number of cells is extremely low (~200 cells). We believe that the developed biochip can be used as a standalone unit in a biology/clinical lab for trapping WBCs as well as other cell types and imaging them using a standard fluorescent microscope at the single cell level. Furthermore, it can be integrated with other miniaturized optical modules to construct a portable platform for counting a wide variety of cells and therefore it can be an excellent tool for monitoring human diseases at the point-of-care.
我们展示了一种用于捕获单个白细胞（WBC）的微流控生物芯片。这种新型生物芯片采用标准的表面微加工工艺进行微制造，由一系列精确设计的微孔组成，这些微孔将单个细胞限制在一个紧密的三维空间中并使其机械固定。当含白细胞的样品以3 psi的最佳压力输送到生物芯片时，实现了高（> 87%）捕获效率。该生物芯片能够高效捕获多达7500个细胞，即使细胞数量极低（约200个细胞）时也能保持高捕获效率。我们认为，所开发的生物芯片可作为生物学/临床实验室中的独立单元，用于捕获白细胞以及其他细胞类型，并使用标准荧光显微镜在单细胞水平对其进行成像。此外，它可以与其他小型化光学模块集成，构建一个用于计数各种细胞的便携式平台，因此它可以成为即时护理点监测人类疾病的出色工具。
