Giorgi M C C, Hernandes N M A P, Sugii M M, Ambrosano G M B, Marchi G M, Lima D A N L, Aguiar F H B
Oper Dent. 2014 May-Jun;39(3):301-7. doi: 10.2341/12-210-L. Epub 2013 Aug 12.
The aim of this in vitro study was to qualitatively and quantitatively evaluate the microleakage of Class II cavities restored with a methacrylate-based composite (Filtek Z250, 3M ESPE) or silorane-based composite (Filtek LS, 3M ESPE), varying the application of an intermediary base, using a low-viscosity composite resin (Filtek Z350 Flow, 3M ESPE) or resin-modified glass ionomer cement (RMGIC) (Vitrebond, 3M ESPE) and no intermediary base (control groups). Sixty cavities were prepared on the proximal surfaces of bovine teeth and were randomly divided according to the experimental groups (n=10). Following the restorative procedures and thermocycling, the samples were immersed in methylene blue for two hours. The qualitative evaluation was made using a stereomicroscope, whereby two observers analyzed the infiltration level of the dye within the tooth/filling. Microleakage scores among the groups were compared using the Kruskal-Wallis test followed by the Mann-Whitney test (p≤0.05). The samples were then ground and the powder was prepared for quantitative analysis in an absorbance spectrophotometer. The results were statistically analyzed by analysis of variance and the Tukey test (p≤0.05). Results from the quantitative analysis showed that LS presented higher values of microleakage than did Z250. There was a significant difference between both composites concerning the intermediary materials, with the lowest values obtained using RMGIC as an intermediary base. Results from the qualitative analysis showed that there were no statistically significant differences between composites; however, there were significant differences for both composites concerning the intermediary materials, with the lowest values obtained using RMGIC as an intermediary. It is possible to conclude that using RMGIC as an intermediary base provided lower microleakage, indicating better sealing of the tooth-restoration interface.
本体外研究的目的是定性和定量评估用甲基丙烯酸酯基复合材料(Filtek Z250,3M ESPE)或硅氧烷基复合材料(Filtek LS,3M ESPE)修复的Ⅱ类洞的微渗漏情况,通过改变中间基底的应用,使用低粘度复合树脂(Filtek Z350 Flow,3M ESPE)或树脂改性玻璃离子水门汀(RMGIC)(Vitrebond,3M ESPE),以及不使用中间基底(对照组)。在牛牙的近中面制备60个洞,并根据实验组(n = 10)随机分组。在进行修复程序和热循环后,将样本浸入亚甲蓝中两小时。使用体视显微镜进行定性评估,由两名观察者分析染料在牙齿/充填物内的渗透水平。使用Kruskal-Wallis检验,随后进行Mann-Whitney检验(p≤0.05)比较各组间的微渗漏评分。然后将样本磨碎并制备粉末,用于在吸收分光光度计中进行定量分析。通过方差分析和Tukey检验(p≤0.05)对结果进行统计学分析。定量分析结果表明,LS的微渗漏值高于Z250。两种复合材料在中间材料方面存在显著差异,以RMGIC作为中间基底时获得的值最低。定性分析结果表明,复合材料之间无统计学显著差异;然而,两种复合材料在中间材料方面存在显著差异,以RMGIC作为中间材料时获得的值最低。可以得出结论,使用RMGIC作为中间基底可降低微渗漏,表明牙齿修复界面的密封效果更好。