Giaccone G, Verga L, Finazzi M, Pollo B, Tagliavini F, Frangione B, Bugiani O
Istituto neurologico Carlo Besta, Milan, Italy.
Neurosci Lett. 1990 Jul 3;114(2):178-83. doi: 10.1016/0304-3940(90)90068-k.
The brains of 7 dogs aged 6 to 18 years have been histochemically and immunohistochemically investigated at the light- and electron microscopy levels for preamyloid deposits and amyloid fibrils to verify the hypothesis that the accumulation of cleavage products of amyloid precursor protein is related not only to Alzheimer's disease but also to the normal aging of the brain. Preamyloid deposits were detected in the neuropil of the cerebral cortex and neostriatum, whereas amyloid fibrils were found in the walls of parenchimal and leptomeningeal vessels. The densities of preamyloid deposits in the neuropil and of deposits of amyloid fibrils in the vessel walls were higher in the brains of the most aged dogs. These findings suggest that aging of the canine brain is characterized by an accumulation of intermediate cleavage products of the amyloid precursor protein in both the neuropil and the vessel walls, and by processing of these products to amyloid fibrils in the vessel walls.
对7只年龄在6至18岁的犬类大脑进行了组织化学和免疫组织化学研究，在光学和电子显微镜水平上检测淀粉样前体沉积物和淀粉样纤维，以验证以下假设：淀粉样前体蛋白裂解产物的积累不仅与阿尔茨海默病有关，还与大脑的正常衰老有关。在大脑皮层和新纹状体的神经纤维网中检测到淀粉样前体沉积物，而在实质和软脑膜血管壁中发现了淀粉样纤维。在年龄最大的犬类大脑中，神经纤维网中淀粉样前体沉积物的密度以及血管壁中淀粉样纤维沉积物的密度更高。这些发现表明，犬类大脑衰老的特征是神经纤维网和血管壁中淀粉样前体蛋白中间裂解产物的积累，以及这些产物在血管壁中加工成淀粉样纤维。
