National Nanotechnology Laboratory, Al-Farabi Kazakh National University, Almaty 050038, Kazakhstan. a
Biomed Res Int. 2013;2013:902467. doi: 10.1155/2013/902467. Epub 2013 Jul 16.
Only PTPN12, MSH6, and ZEB1 have significant miR-1279 binding sites among paralogous genes of human tyrosine phosphatase family, DNA mismatch repair family, and zinc finger family, respectively. All miRNA binding sites are located within CDSs of studied mRNAs. Nucleotide sequences of hsa-miR-1279 binding sites with mRNAs of human PTPN12, MSH6, and ZEB1 genes encode TKEQYE, EGSSDE, and GEKPYE oligopeptides, respectively. The conservation of miRNA binding sites encoding oligopeptides has been revealed. MRNAs of many paralogs of zinc finger gene family have from 1 to 12 binding sites coding the same GEKPYE hexapeptide. MRNAs of PTPN12, MSH6, and ZEB1 orthologous genes from different animal species have binding sites for hsa-miR-1279 which consist of homologous oligonucleotides encoding similar human oligopeptides TKEQYE, EGSSDE, and GEKPYE. MiR-548j, miR-548m, and miR-548d-5p have homologous binding sites in the mRNA of PTPN12 orthologous genes which encode PRTRSC, TEATDI, and STASAT oligopeptides, respectively. All regions of miRNA are important for binding with the mRNA.
只有 PTPN12、MSH6 和 ZEB1 的人类酪氨酸磷酸酶家族、DNA 错配修复家族和锌指家族的同源基因中具有显著的 miR-1279 结合位点。所有 miRNA 结合位点均位于研究的 mRNA 的 CDS 内。hsa-miR-1279 结合位点与人类 PTPN12、MSH6 和 ZEB1 基因的 mRNA 的核苷酸序列分别编码 TKEQYE、EGSSDE 和 GEKPYE 寡肽。已经揭示了编码寡肽的 miRNA 结合位点的保守性。锌指基因家族的许多同源基因的 mRNA 具有 1 到 12 个编码相同的 GEKPYE 六肽的结合位点。来自不同动物物种的 PTPN12、MSH6 和 ZEB1 直系同源基因的 mRNA 具有与 hsa-miR-1279 的结合位点，该结合位点由编码类似人类寡肽 TKEQYE、EGSSDE 和 GEKPYE 的同源寡核苷酸组成。miR-548j、miR-548m 和 miR-548d-5p 在 PTPN12 直系同源基因的 mRNA 中具有同源结合位点，分别编码 PRTRSC、TEATDI 和 STASAT 寡肽。miRNA 的所有区域对于与 mRNA 的结合都是重要的。
