Ivashchenko Anatoly, Berillo Olga, Pyrkova Anna, Niyazova Raigul
National Nanotechnology Laboratory, Al-Farabi Kazakh National University, Al-Farabi 71, Almaty 050038, Kazakhstan.
Biomed Res Int. 2014;2014:620530. doi: 10.1155/2014/620530. Epub 2014 Aug 26.
This study examined binding sites of 2,578 miRNAs in the mRNAs of 12,175 human genes using the MirTarget program. It found that the miRNAs of miR-1273 family have between 33 and 1,074 mRNA target genes, with a free hybridization energy of 90% or more of its maximum value. The miR-1273 family consists of miR-1273a, miR-1273c, miR-1273d, miR-1273e, miR-1273f, miR-1273g-3p, miR-1273g-5p, miR-1273h-3p, and miR-1273h-5p. Unique miRNAs (miR-1273e, miR-1273f, and miR-1273g-3p) have more than 400 target genes. We established 99 mRNA nucleotide sequences that contain arranged binding sites for the miR-1273 family. High conservation of each miRNA binding site in the mRNA of the target genes was found. The arranged binding sites of the miR-1273 family are located in the 5'UTR, CDS, or 3'UTR of many mRNAs. Five repeating sites containing some of the miR-1273 family's binding sites were found in the 3'UTR of several target genes. The oligonucleotide sequences of miR-1273 binding sites located in CDSs code for homologous amino acid sequences in the proteins of target genes. The biological role of unique miRNAs was also discussed.
本研究使用MirTarget程序检测了12175个人类基因的mRNA中2578种miRNA的结合位点。研究发现，miR - 1273家族的miRNA具有33至1074个mRNA靶基因，其自由杂交能达到最大值的90%或更高。miR - 1273家族由miR - 1273a、miR - 1273c、miR - 1273d、miR - 1273e、miR - 1273f、miR - 1273g - 3p、miR - 1273g - 5p、miR - 1273h - 3p和miR - 1273h - 5p组成。独特的miRNA（miR - 1273e、miR - 1273f和miR - 1273g - 3p）具有400多个靶基因。我们建立了99个包含miR - 1273家族排列结合位点的mRNA核苷酸序列。发现靶基因mRNA中每个miRNA结合位点具有高度保守性。miR - 1273家族的排列结合位点位于许多mRNA的5'UTR、CDS或3'UTR中。在几个靶基因的3'UTR中发现了五个包含一些miR - 1273家族结合位点的重复位点。位于CDS中的miR - 1273结合位点的寡核苷酸序列编码靶基因蛋白质中的同源氨基酸序列。还讨论了独特miRNA的生物学作用。
