De Luca M, Tamura R N, Kajiji S, Bondanza S, Rossino P, Cancedda R, Marchisio P C, Quaranta V
Laboratorio di Differenziamento Cellulare, IST, Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy.
Proc Natl Acad Sci U S A. 1990 Sep;87(17):6888-92. doi: 10.1073/pnas.87.17.6888.
Epithelial cell interactions with matrices are critical to tissue organization. Indirect immunofluorescence and immunoprecipitations of cell lysates prepared from stratified cultures of human epidermal cells showed that the major integrins expressed by keratinocytes are alpha E beta 4 (also called alpha 6 beta 4) and alpha 2 beta 1. The alpha E beta 4 integrin is localized at the surface of basal cells in contact with the basement membrane, whereas alpha 2 beta 1/alpha 3 beta 1 integrins are absent from the basal surface and are localized only on the lateral surface of basal and spinous keratinocytes. Anti-beta 4 antibodies potently inhibited keratinocyte adhesion to matrigel or purified laminin, whereas anti-beta 1 antibodies were ineffective. Only anti-beta 4 antibodies were able to detach established keratinocyte colonies. These data suggest that alpha E beta 4 mediates keratinocyte adhesion to basal lamina, whereas the beta 1 subfamily is involved in cell-cell adhesion of keratinocytes.
上皮细胞与基质的相互作用对组织构建至关重要。对从人表皮细胞分层培养物制备的细胞裂解物进行间接免疫荧光和免疫沉淀分析表明，角质形成细胞表达的主要整合素是αEβ4（也称为α6β4）和α2β1。αEβ4整合素定位于与基底膜接触的基底细胞表面，而α2β1/α3β1整合素在基底表面不存在，仅定位于基底和棘层角质形成细胞的侧面。抗β4抗体能有效抑制角质形成细胞与基质胶或纯化层粘连蛋白的黏附，而抗β1抗体则无效。只有抗β4抗体能够使已形成的角质形成细胞集落脱离。这些数据表明，αEβ4介导角质形成细胞与基底层的黏附，而β1亚家族参与角质形成细胞的细胞间黏附。
