Heuer Jan F, Crozier Thomas A, Howard Glenn, Quintel Michael
Department of Anaesthesiology, Emergency and Intensive Care Medicine, University Medical Centre Göttingen, Göttingen, Germany.
GMS Hyg Infect Control. 2013 Apr 29;8(1):Doc09. doi: 10.3205/dgkh000209. eCollection 2013.
In March 2010, more than 213 countries worldwide reported laboratory confirmed cases of influenza H1N1 infections with at least 16,813 deaths. In some countries, roughly 10 to 30% of the hospitalized patients were admitted to the ICU and up to 70% of those required mechanical ventilation. The question now arises whether breathing system filters can prevent virus particles from an infected patient from entering the breathing system and passing through the ventilator into the ambient air. We tested the filters routinely used in our institution for their removal efficacy and efficiency for the influenza virus A H1N1 (A/PR/8/34).
Laboratory investigation of three filters (PALL Ultipor(®) 25, Ultipor(®) 100 and Pall BB50T Breathing Circuit Filter, manufactured by Pall Life Sciences) using a monodispersed aerosol of human influenza A (H1N1) virus in an air stream model with virus particles quantified as cytopathic effects in cultured canine kidney cells (MDCK).
The initial viral load of 7.74±0.27 log10 was reduced to a viral load of ≤2.43 log10, behind the filter. This represents a viral filtration efficiency of ≥99.9995%.
The three tested filters retained the virus input, indicating that their use in the breathing systems of intubated and mechanically ventilated patients can reduce the risk of spreading the virus to the breathing system and the ambient air.
2010年3月，全球213多个国家报告了实验室确诊的甲型H1N1流感感染病例，至少16,813人死亡。在一些国家，约10%至30%的住院患者被收入重症监护病房，其中高达70%的患者需要机械通气。现在的问题是，呼吸系统过滤器能否防止感染患者的病毒颗粒进入呼吸系统，并通过呼吸机进入周围空气。我们测试了本机构常规使用的过滤器对甲型H1N1流感病毒（A/PR/8/34）的去除效果和效率。
在气流模型中，使用人甲型（H1N1）流感病毒的单分散气溶胶对三种过滤器（颇尔生命科学公司生产的颇尔Ultipor(®) 25、Ultipor(®) 100和颇尔BB50T呼吸回路过滤器）进行实验室研究，病毒颗粒在培养的犬肾细胞（MDCK）中以细胞病变效应进行定量。
过滤器后，初始病毒载量7.74±0.27 log10降至病毒载量≤2.43 log10。这代表病毒过滤效率≥99.9995%。
所测试的三种过滤器均能截留输入的病毒，表明在插管和机械通气患者的呼吸系统中使用这些过滤器可降低病毒传播至呼吸系统和周围空气的风险。
