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5-二甲基氨基萘-1-磺酰基-糖肽的电泳迁移率及其与分子量的关系

The electrophoretic mobilities of 5-dimethylaminoaphthalene-1-suphonyl-glycopeptides and their relation molecular weight.

作者信息

Wenn R V

出版信息

Biochem J. 1975 Feb;145(2):281-5. doi: 10.1042/bj1450281.

DOI:10.1042/bj1450281
PMID:239681
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1165216/
Abstract

The relationship between the electrophoretic mobility at pH2.1 of dansyl-glycopeptides of known composition and their molecular weight is shown to conform with a model equation previously derived for peptides. A dansyl-glycopeptide prepared from hen's-egg ovotransferrin is degraded sequentially with two glycosidases. The molecular weight of each glycopeptide intermediate formed is determined from its electrophoretic mobility. From successive molecular-weight changes, the number and type of sugar residues lost from the parent glycopeptide can be decided and the probable composition of each intermediate determined. The notion that the method has considerable application and would permit analysis of very small quantities of glycopeptides is discussed.

摘要

已知组成的丹磺酰 - 糖肽在pH2.1时的电泳迁移率与其分子量之间的关系表明符合先前为肽推导的模型方程。从鸡卵转铁蛋白制备的丹磺酰 - 糖肽依次用两种糖苷酶降解。通过其电泳迁移率确定形成的每种糖肽中间体的分子量。根据连续的分子量变化，可以确定从母体糖肽中丢失的糖残基的数量和类型，并确定每种中间体的可能组成。讨论了该方法具有广泛应用并允许分析极少量糖肽的观点。

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本文引用的文献

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Reagent for Differentiation of 1,4- and 1,6-Linked Glucosaccharides.用于区分1,4-和1,6-连接的葡萄糖苷的试剂。
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Studies on glucosaminidase. 2. Substrates for N-acetyl-beta-glucosaminidase.氨基葡萄糖苷酶研究。2. N-乙酰-β-氨基葡萄糖苷酶的底物。
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THE AMINO ACID SEQUENCE OF PSEUDOMONAS CYTOCHROME C-551.铜绿假单胞菌细胞色素C-551的氨基酸序列。
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Inhibition of glycosidases by aldonolactones of corresponding configuration. 2. Inhibitors of beta-N-acetylglucosaminidase.相应构型的醛糖内酯对糖苷酶的抑制作用。2. β-N-乙酰氨基葡萄糖苷酶的抑制剂。
Biochem J. 1958 Jul;69(3):467-76. doi: 10.1042/bj0690467.
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The determination of glucosamine and galactosamine.葡萄糖胺和半乳糖胺的测定
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Electrophoretic mobilities of peptides on paper and their use in the determination of amide groups.肽在纸上的电泳迁移率及其在酰胺基团测定中的应用。
Nature. 1966 Aug 6;211(5049):591-3. doi: 10.1038/211591a0.
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A comparison of glycopeptides from the ovotransferrin and serum transferrin of the hen.母鸡卵转铁蛋白和血清转铁蛋白中糖肽的比较。
Biochem J. 1968 Jun;108(1):57-67. doi: 10.1042/bj1080057.
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The electrophoretic mobility of peptides on paper at pH 1.9.在pH值为1.9时，肽在纸上的电泳迁移率。
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