Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacky University, Hněvotínská 3, 775 15 Olomouc, Czech Republic.
J Photochem Photobiol B. 2013 Nov 5;128:1-11. doi: 10.1016/j.jphotobiol.2013.07.024. Epub 2013 Aug 8.
UVA photons are less energetic than UVB photons but they are more abundant in solar radiation. Modern tools have shown that UVA light has serious adverse effects on the skin. We investigated the effect of consuming Lonicera caerulea berries on UVA-induced damage in SKH-1 mice. The mice were fed a diet containing L. caerulea berries (10%, w/w) for 14 days before a single UVA (30 J/cm(2)) treatment. Effects on haematological and antioxidant parameters were evaluated 4 and 24h after irradiation. The bioavailability of L. caerulea phenolics was also assessed. Consuming the L. caerulea berry-enriched diet caused reduced malondialdehyde production and increased catalase activity and glutathione levels were found in skin and erythrocytes. UVA-induced NADPH:quinone oxidoreductase-1 and gamma-L-glutamate-L-cysteine ligase protein in skin were reduced in mice fed L. caerulea berries. Enhanced heme oxygenase-1 level in skin, interleukin-17 in plasma and reduced interleukin-12 levels in plasma were found in the mice on the experimental diet. Histological (pyknotic) changes in the nuclei of basal cells induced by UVA exposure were reduced in L. caerulea berry consuming animals. HLPC-MS analysis showed high concentrations of hippuric acid, one of the main metabolites of aromatic amino acids and phenolic compounds, in skin, liver, urine and faeces of mice consuming the berries. Taken together, consumption of L. caerulea berries affords protection from the adverse effects of a single UVA exposure mainly via modulation of antioxidant parameters.
UVA 光子的能量低于 UVB 光子，但在太阳辐射中更为丰富。现代工具表明，UVA 光对皮肤有严重的不良影响。我们研究了食用金银花浆果对 SKH-1 小鼠 UVA 诱导损伤的影响。在单次 UVA（30 J/cm²）处理前，小鼠连续 14 天喂食含金银花浆果（10%，w/w）的饮食。照射后 4 和 24 小时评估血液学和抗氧化参数的影响。还评估了金银花酚类化合物的生物利用度。食用富含金银花浆果的饮食会导致丙二醛产生减少，皮肤和红细胞中的过氧化氢酶活性增加，谷胱甘肽水平升高。在喂食金银花浆果的小鼠中，UVA 诱导的 NADPH：醌氧化还原酶-1 和γ-L-谷氨酰-L-半胱氨酸连接酶蛋白在皮肤中减少。在实验饮食的小鼠中，皮肤中血红素加氧酶-1 水平升高，血浆中白细胞介素-17 降低，血浆中白细胞介素-12 水平降低。暴露于 UVA 后，基底细胞细胞核中的核固缩（pyknotic）变化在食用金银花浆果的动物中减少。HLPC-MS 分析显示，在食用浆果的小鼠的皮肤、肝脏、尿液和粪便中，芳香族氨基酸和酚类化合物的主要代谢物之一马尿酸的浓度很高。总之，食用金银花浆果可通过调节抗氧化参数来防止单次 UVA 暴露的不良影响。
