一株犬中间葡萄球菌分离株中新的 fexA 变异体，该变异体不赋予氟苯尼考耐药性。
A novel fexA variant from a canine Staphylococcus pseudintermedius isolate that does not confer florfenicol resistance.
机构信息
Biochemistry and Molecular Biology, University of La Rioja, Logroño, Spain.
出版信息
Antimicrob Agents Chemother. 2013 Nov;57(11):5763-6. doi: 10.1128/AAC.00948-13. Epub 2013 Aug 26.
Abstract
Transposon Tn558 integrated in the chromosomal radC gene was detected for the first time in Staphylococus pseudintermedius. It carried a novel fexA variant (fexAv) that confers only chloramphenicol resistance. The exporter FexAv exhibited two amino acid substitutions, Gly33Ala and Ala37Val, both of which seem to be important for substrate recognition. Site-directed mutagenesis that reverted the mutated base pairs to those present in the original fexA gene restored the chloramphenicol-plus-florfenicol resistance phenotype.
摘要
转座子 Tn558 首次在金黄色葡萄球菌中间亚种中整合到染色体 radC 基因中。它携带一种新型的 fexA 变体（fexAv），仅赋予氯霉素抗性。该外排泵 FexAv 表现出两种氨基酸取代，甘氨酸 33 突变为丙氨酸和丙氨酸 37 突变为缬氨酸，这两种取代似乎对底物识别很重要。将突变碱基对回复为原始 fexA 基因中存在的碱基对的定点突变，恢复了氯霉素加氟苯尼考的抗性表型。
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