Dhakar Nidhi, Astekar Madhusudan, Jain Mahesh, Saawarn Swati, Saawarn Nisheeth
Department of Oral Pathology and Microbiology, Darshan Dental College and Hospital, Udaipur, India.
Dent Res J (Isfahan). 2013 May;10(3):343-7. doi: 10.4103/1735-3327.115155.
Detection of cancer at an early stage is of utmost importance to decrease the morbidity and mortality of the disease. Apart from the conventional biopsy, non-invasive methods like analysis of serum and saliva may provide cost-effective approach for screening a large population. Tumor markers are a major part of secondary prevention and thus, the detection of malignancies. The aim of this study was to evaluate total sialic acid (TSA), total protein and total sugar (TS) in serum and saliva of oral squamous cell carcinoma (OSCC) and controls to assess their role as a diagnostic marker.
Unstimulated whole saliva and sera were collected from 40 squamous cell carcinoma patients and 20 controls. Serum and salivary TSA, total protein and TS estimation was carried out. This was correlated with clinical stages and histopathological grades of OSCC. The data obtained was analyzed statistically using Chi-square test, ANOVA and Student's t-test with SPSS statistical software.
A highly significant rise in the salivary sialic acid, serum sialic acid and serum protein was noted in OSCC subjects compared to controls. Salivary protein, serum and salivary sugar did not show any significance. Furthermore, serum and salivary sialic acid levels were found to be significantly increased with increasing level of histopathological grading.
The present study showed a significant increase in serum sialic acid, salivary sialic acid and serum protein from control to OSCC and suggests that these markers may be reliable in diagnosis and predicting prognosis.
早期发现癌症对于降低该疾病的发病率和死亡率至关重要。除了传统的活检外，血清和唾液分析等非侵入性方法可能为大规模人群筛查提供经济有效的途径。肿瘤标志物是二级预防的重要组成部分，因此也是恶性肿瘤检测的重要组成部分。本研究的目的是评估口腔鳞状细胞癌（OSCC）患者和对照组血清及唾液中的总唾液酸（TSA）、总蛋白和总糖（TS），以评估它们作为诊断标志物的作用。
收集40例鳞状细胞癌患者和20例对照者的非刺激性全唾液和血清。进行血清和唾液TSA、总蛋白及TS的测定。将其与OSCC的临床分期和组织病理学分级相关联。使用SPSS统计软件，通过卡方检验、方差分析和学生t检验对获得的数据进行统计学分析。
与对照组相比，OSCC患者唾液唾液酸、血清唾液酸和血清蛋白显著升高。唾液蛋白、血清和唾液糖无显著差异。此外，血清和唾液唾液酸水平随组织病理学分级升高而显著增加。
本研究表明，从对照组到OSCC患者，血清唾液酸、唾液唾液酸和血清蛋白显著增加，提示这些标志物在诊断和预测预后方面可能是可靠的。
