DePaola A, Hopkins L H, Peeler J T, Wentz B, McPhearson R M
Fishery Research Branch, U.S. Food and Drug Administration, Dauphin Island, Alabama 36528.
Appl Environ Microbiol. 1990 Aug;56(8):2299-302. doi: 10.1128/aem.56.8.2299-2302.1990.
Oyster and seawater samples were collected seasonally from May 1984 through April 1985 from shellfish-growing areas in Washington, California, Texas, Louisiana, Alabama, Florida, South Carolina, Virginia, and Rhode Island which had been designated as approved or prohibited by the National Shellfish Sanitation Program. Fecal coliforms counts, aerobic plate counts, and Vibrio parahaemolyticus densities were determined for the samples. Mean V. parahaemolyticus density was more than 100 times greater in oysters than in water, whereas density of fecal coliforms was approximately 10 times higher in oysters. Seasonal and geographical distributions of V. parahaemolyticus were related to water temperature, with highest densities in samples collected in the spring and the summer along the Gulf coast. The synthetic DNA probe for thermostable direct hemolysin hybridized with 2 of 50 isolates, 1 of which was positive by the Kanagawa test.
1984年5月至1985年4月期间，从华盛顿、加利福尼亚、得克萨斯、路易斯安那、亚拉巴马、佛罗里达、南卡罗来纳、弗吉尼亚和罗德岛的贝类养殖区季节性采集牡蛎和海水样本，这些地区已被国家贝类卫生计划指定为批准或禁止区域。对样本进行了粪大肠菌群计数、需氧平板计数和副溶血性弧菌密度测定。牡蛎中副溶血性弧菌的平均密度比水中高100多倍，而牡蛎中粪大肠菌群的密度约高10倍。副溶血性弧菌的季节性和地理分布与水温有关，春季和夏季在墨西哥湾沿岸采集的样本中密度最高。用于耐热直接溶血素的合成DNA探针与50株分离株中的2株杂交，其中1株经神奈川试验呈阳性。
