Overman T L, Kessler J F, Seabolt J P
J Clin Microbiol. 1985 Nov;22(5):778-81. doi: 10.1128/jcm.22.5.778-781.1985.
Sixty isolates, from nine species of the family Vibrionaceae, were tested by the API 20E, API Rapid E, and API Rapid NFT systems. Results were compared with those obtained with standard biochemicals. Included were 7 Aeromonas caviae isolates, 27 Aeromonas hydrophilia isolates, 10 Aeromonas sobria isolates, 3 Plesiomonas shigelloides isolates, 3 Vibrio alginolyticus isolates, 3 Vibrio cholerae isolates, 1 Vibrio fluvialis isolate, 5 Vibrio parahaemolyticus isolates, and 1 Vibrio vulnificus isolate. The API 20E correctly identified all the isolates within 24 h. The API Rapid E correctly identified only 77%, misidentified 8%, and failed to identify 2% of the isolates in 4 h. The remaining 13% of the isolates gave a low selectivity identification, with one of the choices being correct. The API Rapid NFT correctly identified 87%, misidentified 5%, gave a low selectivity identification for 8% of the isolates, and in some instances, required up to 48 h of incubation. The API 20E is a valid system for use in the identification of the more commonly occurring members of the family Vibrionaceae and the most accurate and efficient of the three systems tested.
对弧菌科9个菌种的60株分离菌，采用API 20E、API Rapid E和API Rapid NFT系统进行检测。将结果与用标准生化试剂检测获得的结果进行比较。其中包括7株豚鼠气单胞菌分离菌、27株嗜水气单胞菌分离菌、10株温和气单胞菌分离菌、3株类志贺邻单胞菌分离菌、3株溶藻弧菌分离菌、3株霍乱弧菌分离菌、1株河弧菌分离菌、5株副溶血性弧菌分离菌和1株创伤弧菌分离菌。API 20E在24小时内正确鉴定了所有分离菌。API Rapid E在4小时内仅正确鉴定了77%的分离菌，错误鉴定了8%，未能鉴定2%的分离菌。其余13%的分离菌给出了低选择性鉴定结果，其中一个选择是正确的。API Rapid NFT正确鉴定了87%，错误鉴定了5%，对8%的分离菌给出了低选择性鉴定结果，在某些情况下，需要长达48小时的培养。API 20E是用于鉴定弧菌科较常见成员的有效系统，也是所测试的三个系统中最准确、最有效的。
