用于检测神奈川现象阳性副溶血性弧菌的合成寡脱氧核糖核苷酸探针。
Synthetic oligodeoxyribonucleotide probes to detect Kanagawa phenomenon-positive Vibrio parahaemolyticus.
作者信息
Nishibuchi M, Hill W E, Zon G, Payne W L, Kaper J B
出版信息
J Clin Microbiol. 1986 Jun;23(6):1091-5. doi: 10.1128/jcm.23.6.1091-1095.1986.
Abstract
Synthetic oligodeoxyribonucleotide probes were used in the colony hybridization test to examine the association between the Kanagawa phenomenon (KP) and the thermostable direct hemolysin gene (tdh) of Vibrio parahaemolyticus. Representative V. parahaemolyticus strains with a variety of KP reactions and 17 other Vibrio species were examined for homology with four synthetic oligodeoxyribonucleotide probes (19 to 21 bases long) representing different regions of the tdh structural gene. Under stringent conditions, two of the probes were capable of distinguishing KP-positive V. parahaemolyticus from KP-negative or KP weak-positive V. parahaemolyticus which possesses mutated tdh genes. Vibrio hollisae strains hybridized with all four probes under reduced stringency, suggesting that they have tdh-related genes which are homologous but not identical to the tdh gene in all the regions examined. The results suggest that the colony hybridization test with the synthetic oligonucleotide probes is more suitable for the definitive determination of KP-positive strains than the hybridization with the larger gene probe or immunological assays.
摘要
在菌落杂交试验中使用合成的寡聚脱氧核糖核苷酸探针，以检测副溶血性弧菌的神奈川现象（KP）与耐热直接溶血素基因（tdh）之间的关联。对具有各种KP反应的代表性副溶血性弧菌菌株以及其他17种弧菌进行检测，以确定它们与代表tdh结构基因不同区域的四种合成寡聚脱氧核糖核苷酸探针（长度为19至21个碱基）的同源性。在严格条件下，其中两种探针能够区分KP阳性的副溶血性弧菌与具有突变tdh基因的KP阴性或KP弱阳性的副溶血性弧菌。霍利斯弧菌菌株在较低严谨度下与所有四种探针杂交，这表明它们具有与tdh相关的基因，这些基因在所检测的所有区域中与tdh基因同源但不相同。结果表明，与较大基因探针杂交或免疫测定相比，使用合成寡核苷酸探针进行菌落杂交试验更适合于明确确定KP阳性菌株。
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